Human EIF1 Recombinant Protein (C-His) (HD027012)

Name: Human EIF1 Recombinant Protein (C-His)
Brand: abinScience
SKU: HD027012
Price: 211 USD
Availability: InStock

데이터시트 제품 공유 정보 복사

가격(USD):

사양:

50ug
100ug
1mg

수량:

문의하기

개요
이미지
참고 문헌
데이터시트
SDS

개요

카탈로그 번호	HD027012
설명	Recombinant Human EIF1 Protein, C-His (HD027012) expressed in E. coli, spanning Met1-Phe113. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
발현 시스템	E. coli
등록번호	P41567
단백질 길이	Met1-Phe113
응용 분야	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
종	Homo sapiens (Human)
성질	Recombinant
내독소 수준	Please contact with the lab for this information.
순도	>90% as determined by SDS-PAGE.
예측 분자량	13.80 kDa
형태	Lyophilized
저장 완충액	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.
재구성	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
배송	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
안정성 및 저장	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
대체 이름	A121, EIF1, Eukaryotic translation initiation factor 1, Protein translation factor SUI1 homolog, SUI1, Sui1iso1, eIF1
배경	Eukaryotic translation initiation factor 1 is a ~12 kDa protein. Component of the 43S pre-initiation complex (43S PIC), which binds to the mRNA cap-proximal region, scans mRNA 5'-untranslated region, and locates the initiation codon. Together with eIF1A (EIF1AX), EIF1 facilitates scanning and is essential for start codon recognition on the basis of AUG nucleotide context and location relative to the 5'-cap. Participates to initiation codon selection by influencing the conformation of the 40S ribosomal subunit and the positions of bound mRNA and initiator tRNA; this is possible after its binding to the interface surface of the platform of the 40S ribosomal subunit close to the P-site. Together with eIF1A (EIF1AX), also regulates the opening and closing of the mRNA binding channel, which ensures mRNA recruitment, scanning and the fidelity of initiation codon selection. Continuously monitors and protects against premature and partial base-pairing of codons in the 5'-UTR with the anticodon of initiator tRNA. 1. Pestova, TV. et al. (2002) Genes & development 16, 2906-22. PMID: 12435632 2. Lomakin, IB. et al. (2003) Genes & development 17, 2786-97. PMID: 14600024 3. Pestova, TV. et al. (1998) Nature 394, 854-9. PMID: 9732867 4. Haimov, O. et al. (2018) Molecular and cellular biology 38. PMID: 29987188 5. Luna, RE. et al. (2013) Biochemistry 52, 9510-8. PMID: 24319994 6. Luna, RE. et al. (2012) Cell reports 1, 689-702. PMID: 22813744
참고사항	For research use only

이미지

참고 문헌

데이터시트

Human EIF1 Recombinant Protein (C-His).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Human EIF1 Recombinant Protein (C-His) (HD027012)

개요

이미지

참고 문헌

데이터시트

SDS

빠른 접근

문의하기