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| 카탈로그 번호 | HD950012 |
|---|---|
| 설명 |
Recombinant Human FBXW11 Protein, N-His (HD950012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | Q9UKB1 |
| 단백질 길이 | Arg227-Asn521 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | BTRCP2, F-box and WD repeats protein beta-TrCP2, F-box/WD repeat-containing protein 11, F-box/WD repeat-containing protein 1B, FBW1B, FBXW11, FBXW1B, HOS, Homologous to Slimb protein, KIAA0696 |
| 배경 | F-box/WD repeat-containing protein 11 is a ~62 kDa protein. Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins: the interaction with substrates requires the phosphorylation of the two serine residues in the substrates' destruction motif D-S-G-X(2,3,4)-S. SCF(FBXW11) mediates the ubiquitination of phosphorylated CTNNB1 and participates in Wnt signaling regulation. SCF(FBXW11) plays a key role in NF-kappa-B activation by mediating ubiquitination of phosphorylated NFKBIA, leading to its degradation by the proteasome, thereby allowing the associated NF-kappa-B complex to translocate into the nucleus and to activate transcription. The SCF(FBXW11) complex also regulates NF-kappa-B by mediating ubiquitination of phosphorylated NFKB1: specifically ubiquitinates the p105 form of NFKB1, leading to its degradation. 1. Vuillard, L. et al. (1999) FEBS letters 455, 311-4. PMID: 10437795 2. Wu, K. et al. (2000) Molecular and cellular biology 20, 1382-93. PMID: 10648623 3. Heissmeyer, V. et al. (2001) Molecular and cellular biology 21, 1024-35. PMID: 11158290 4. Peschiaroli, A. et al. (2010) Oncogene 29, 1384-93. PMID: 19966869 5. Wu, K. et al. (2010) Molecular cell 37, 784-96. PMID: 20347421 6. Gao, D. et al. (2011) Molecular cell 44, 290-303. PMID: 22017875 7. Zhao, Y. et al. (2011) Molecular cell 44, 304-16. PMID: 22017876 8. Nardone, C. et al. (2023) Molecular cell 83, 57-73.e9. PMID: 36608670 10. Suzuki, H. et al. (2000) The Journal of biological chemistry 275, 2877-84. PMID: 10644755 |
| 참고사항 | For research use only |
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