Human FTO Recombinant Protein (C-His) (HP668011)

Name: Human FTO Recombinant Protein (C-His)
Brand: abinScience
SKU: HP668011
Price: 298 USD
Availability: InStock

데이터시트 제품 공유 정보 복사

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사양:

50ug
100ug
1mg

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개요
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참고 문헌
데이터시트
SDS

개요

카탈로그 번호	HP668011
설명	Recombinant Human FTO Protein, C-His (HP668011) expressed in Mammalian Cells, spanning Met1-Pro505. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●Mammalian Expression — Proper folding and native glycosylation. ●High Purity — >90% purity verified by SDS-PAGE.
발현 시스템	Mammalian cells
등록번호	Q9C0B1
단백질 길이	Met1-Pro505
응용 분야	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
종	Homo sapiens (Human)
성질	Recombinant
내독소 수준	Please contact with the lab for this information.
순도	>90% as determined by SDS-PAGE.
형태	Lyophilized
저장 완충액	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.
재구성	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
배송	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
안정성 및 저장	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
대체 이름	Alpha-ketoglutarate-dependent dioxygenase FTO, Fat mass and obesity-associated protein, U6 small nuclear RNA (2'-O-methyladenosine-N(6)-)-demethylase FTO, 1.14.11.-, U6 small nuclear RNA N(6)-methyladenosine-demethylase FTO, 1.14.11.-, mRNA (2'-O-methyladenosine-N(6)-)-demethylase FTO, m6A(m)-demethylase FTO, 1.14.11.-, mRNA N(6)-methyladenosine demethylase FTO, 1.14.11.53, tRNA N1-methyl adenine demethylase FTO, 1.14.11.-, FTO, KIAA1752
배경	Alpha-ketoglutarate-dependent dioxygenase FTO is a ~58 kDa protein. RNA demethylase that mediates oxidative demethylation of different RNA species, such as mRNAs, tRNAs and snRNAs, and acts as a regulator of fat mass, adipogenesis and energy homeostasis. Specifically demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes. M6A demethylation by FTO affects mRNA expression and stability. Also able to demethylate m6A in U6 small nuclear RNA (snRNA). Mediates demethylation of N(6),2'-O-dimethyladenosine cap (m6A(m)), by demethylating the N(6)-methyladenosine at the second transcribed position of mRNAs and U6 snRNA. _x005F_x005F_x000D_ _x005F_x005F_x000D_ 1. Jia, G. et al. (2011) Nature chemical biology 7, 885-7. PMID: 22002720 _x005F_x005F_x000D_ _x005F_x005F_x000D_ 2. Huang, Y. et al. (2015) Nucleic acids research 43, 373-84. PMID: 25452335 _x005F_x005F_x000D_ _x005F_x005F_x000D_ 3. Wang, T. et al. (2015) Journal of the American Chemical Society 137, 13736-9. PMID: 26457839 _x005F_x005F_x000D_ _x005F_x005F_x000D_ 4. Zhou, J. et al. (2015) Nature 526, 591-4. PMID: 26458103 _x005F_x005F_x000D_ _x005F_x005F_x000D_ 5. Mauer, J. et al. (2017) Nature 541, 371-375. PMID: 28002401 _x005F_x005F_x000D_ _x005F_x005F_x000D_ 6. Wei, J. et al. (2018) Molecular cell 71, 973-985.e5. PMID: 30197295 _x005F_x005F_x000D_
참고사항	For research use only.

이미지

SDS-PAGE

SDS-PAGE for Human FTO Recombinant Protein (C-His)

참고 문헌

데이터시트

Human FTO Recombinant Protein (C-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Human FTO Recombinant Protein (C-His) (HP668011)

개요

이미지

참고 문헌

데이터시트

SDS

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