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Human GTF2B Recombinant Protein (N-His) (HD836012)

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사양:
  • 50ug
  • 100ug
  • 1mg
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  • 개요

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  • 데이터시트

  • SDS

개요
카탈로그 번호HD836012
설명
Recombinant Human GTF2B Protein, N-His (HD836012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
  • ●E. coli Expression — High-yield, cost-effective production.
  • ●High Purity — >90% as determined by SDS-PAGE.
발현 시스템E. coli
등록번호Q00403
단백질 길이Ser108-Leu316
응용 분야ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
종Homo sapiens (Human)
성질 Recombinant
내독소 수준 Please contact with the lab for this information.
순도 >90% as determined by SDS-PAGE.
형태 Lyophilized
저장 완충액 Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

재구성 Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
배송 In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
안정성 및 저장 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
대체 이름EC:2.3.1.48, GTF2B, General transcription factor TFIIB, S300-II, TF2B, TFIIB, Transcription initiation factor IIB
배경

Transcription initiation factor IIB is a ~34 kDa protein. General transcription factor that plays a role in transcription initiation by RNA polymerase II (Pol II). Involved in the pre-initiation complex (PIC) formation and Pol II recruitment at promoter DNA. Together with the TATA box-bound TBP forms the core initiation complex and provides a bridge between TBP and the Pol II-TFIIF complex. Released from the PIC early following the onset of transcription during the initiation and elongation transition and reassociates with TBP during the next transcription cycle. Associates with chromatin to core promoter-specific regions.

1. Choi, CH. et al. (2003) Nature 424, 965-9. PMID: 12931194
2. Ing, NH. et al. (1992) The Journal of biological chemistry 267, 17617-23. PMID: 1517211
3. Ha, I. et al. (1991) Nature 352, 689-95. PMID: 1876184
4. Malik, S. et al. (1991) Proceedings of the National Academy of Sciences of the United States of America 88, 9553-7. PMID: 1946368
5. He, Y. et al. (2016) Nature 533, 359-65. PMID: 27193682
6. Reinberg, D. et al. (1987) The Journal of biological chemistry 262, 3310-21. PMID: 3029109
7. Reinberg, D. et al. (1987) The Journal of biological chemistry 262, 3322-30. PMID: 3818643
8. Zawel, L. et al. (1995) Genes & development 9, 1479-90. PMID: 7601352
9. Malik, S. et al. (1993) Molecular and cellular biology 13, 6253-9. PMID: 8413225
10. Hisatake, K. et al. (1993) Nature 363, 744-7. PMID: 8515820
참고사항 For research use only
이미지
참고 문헌
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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