Human HLA-A Recombinant Protein (N-His) (HM834022)

Name: Human HLA-A Recombinant Protein (N-His)
Brand: abinScience
SKU: HM834022
Price: 211 USD
Availability: InStock

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50ug
100ug
1mg

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개요
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참고 문헌
데이터시트
SDS

개요

카탈로그 번호	HM834022
설명	Recombinant Human HLA-A Protein, N-His (HM834022) expressed in E. coli, spanning Gly25-Leu300. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
발현 시스템	E. coli
등록번호	P04439
단백질 길이	Gly25-Leu300
응용 분야	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
종	Homo sapiens (Human)
성질	Recombinant
내독소 수준	Please contact with the lab for this information.
순도	>90% as determined by SDS-PAGE.
예측 분자량	34.12 kDa
형태	Lyophilized
저장 완충액	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.
재구성	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
배송	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
안정성 및 저장	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
대체 이름	HLAA, Human leukocyte antigen A, HLA class I histocompatibility antigen, A alpha chain, HLA-A
배경	HLA class I histocompatibility antigen, A alpha chain (HLA-A) is a ~40 kDa protein. Antigen-presenting major histocompatibility complex class I (MHCI) molecule. In complex with B2M/beta 2 microglobulin displays primarily viral and tumor-derived peptides on antigen-presenting cells for recognition by alpha-beta T cell receptor (TCR) on HLA-A-restricted CD8-positive T cells, guiding antigen-specific T cell immune response to eliminate infected or transformed cells. May also present self-peptides derived from the signal sequence of secreted or membrane proteins, although T cells specific for these peptides are usually inactivated to prevent autoreactivity. Both the peptide and the MHC molecule are recognized by TCR, the peptide is responsible for the fine specificity of antigen recognition and MHC residues account for the MHC restriction of T cells. Typically presents intracellular peptide antigens of 8 to 13 amino acids that arise from cytosolic proteolysis via IFNG-induced immunoproteasome or via endopeptidase IDE/insulin-degrading enzyme. 1. Fukada, K. et al. (1999) AIDS (London, England) 13, 1413-4. PMID: 10449296 2. Nagata, Y. et al. (2002) Proceedings of the National Academy of Sciences of the United States of America 99, 10629-34. PMID: 12138174 3. Kuzushima, K. et al. (2003) Blood 101, 1460-8. PMID: 12393434 4. Traversari, C. et al. (1992) The Journal of experimental medicine 176, 1453-7. PMID: 1402688 5. Satoh, M. et al. (2005) Vaccine 23, 3783-90. PMID: 15893615 6. Asemissen, AM. et al. (2006) Clinical cancer research : an official journal of the American Association for Cancer Research 12, 7476-82. PMID: 17189421 7. Hadrup, SR. et al. (2009) Nature methods 6, 520-6. PMID: 19543285 8. Matthews, PC. et al. (2011) Journal of immunology (Baltimore, Md. : 1950) 186, 5675-86. PMID: 21498667 9. Shimizu, A. et al. (2013) Scientific reports 3, 3097. PMID: 24192765 10. Quiñones-Parra, S. et al. (2014) Proceedings of the National Academy of Sciences of the United States of America 111, 1049-54. PMID: 24395804
참고사항	For research use only.