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| 카탈로그 번호 | HD661012 |
|---|---|
| 설명 |
Recombinant Human HTATSF1 Protein, N-His (HD661012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | O43719 |
| 단백질 길이 | Phe125-Ser389 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | 17S U2 SnRNP complex component HTATSF1, HIV Tat-specific factor 1, HTATSF1, Tat-SF1 |
| 배경 | 17S U2 SnRNP complex component HTATSF1 is a ~85 kDa protein. Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs. The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing. Within the 17S U2 SnRNP complex, HTATSF1 is required to stabilize the branchpoint-interacting stem loop. HTATSF1 is displaced from the 17S U2 SnRNP complex before the stable addition of the 17S U2 SnRNP complex to the spliceosome, destabilizing the branchpoint-interacting stem loop and allowing to probe intron branch site sequences. Also acts as a regulator of transcriptional elongation, possibly by mediating the reciprocal stimulatory effect of splicing on transcriptional elongation. 1. Loerch, S. et al. (2019) The Journal of biological chemistry 294, 2892-2902. PMID: 30567737 2. Zhang, Z. et al. (2020) Nature 583, 310-313. PMID: 32494006 3. Tholen, J. et al. (2022) Science (New York, N.Y.) 375, 50-57. PMID: 34822310 4. Kim, JB. et al. (1999) Molecular and cellular biology 19, 5960-8. PMID: 10454543 |
| 참고사항 | For research use only |
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