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| 카탈로그 번호 | HC458012 |
|---|---|
| 설명 |
Recombinant Human IRF3 Protein, N-His (HC458012) expressed in E. coli, spanning Met1-Ser123. Purity: >90% by SDS-PAGE.
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | Q14653 |
| 단백질 길이 | Met1-Ser123 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 예측 분자량 | 16.29 kDa |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | IRF-3, IRF3, Interferon regulatory factor 3 |
| 배경 | Interferon regulatory factor 3 (IRF3) is a ~47 kDa protein. Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. 1. Tanaka, Y. et al. (2012) Science signaling 5, ra20. PMID: 22394562 2. Wang, S. et al. (2013) Journal of virology 87, 12814-27. PMID: 24049179 3. Liu, S. et al. (2015) Science (New York, N.Y.) 347, aaa2630. PMID: 25636800 4. Zhao, B. et al. (2016) Proceedings of the National Academy of Sciences of the United States of America 113, E3403-12. PMID: 27302953 5. Li, D. et al. (2019) Journal of cell science 133. PMID: 31340999 6. Yang, S. et al. (2023) Molecular cell 83, 298-313.e8. PMID: 36603579 7. Au, WC. et al. (1995) Proceedings of the National Academy of Sciences of the United States of America 92, 11657-61. PMID: 8524823 8. Zhu, H. et al. (2024) Cell death & disease 15, 727. PMID: 39362857 9. Yoneyama, M. et al. (2002) Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research 22, 73-6. PMID: 11846977 10. Solis, M. et al. (2006) Biochemical pharmacology 72, 1469-76. PMID: 16846591 |
| 참고사항 | For research use only. |
SDS-PAGE for Recombinant Human IRF3 Protein, N-His
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