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| 카탈로그 번호 | HV301012 |
|---|---|
| 설명 |
Recombinant Human KAT5 Protein, N-His (HV301012) expressed in E. coli, spanning Ala101-Ser457. Purity: >90% by SDS-PAGE.
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | Q92993 |
| 단백질 길이 | Ala101-Ser457 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 예측 분자량 | 42.61 kDa |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | Tip60, cPLA(2)-interacting protein, Histone acetyltransferase KAT5, 60 kDa Tat-interactive protein, KAT5, Lysine acetyltransferase 5, HTATIP, TIP60, HIV-1 Tat interactive protein, Histone acetyltransferase HTATIP |
| 배경 | Histone acetyltransferase KAT5 is a ~58 kDa protein. Catalytic subunit of the NuA4 histone acetyltransferase complex, a multiprotein complex involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H2A and H4. Histone acetylation alters nucleosome-DNA interactions and promotes interaction of the modified histones with other proteins which positively regulate transcription. The NuA4 histone acetyltransferase complex is required for the activation of transcriptional programs associated with proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex plays a direct role in repair of DNA double-strand breaks (DSBs) by promoting homologous recombination (HR): the complex inhibits TP53BP1 binding to chromatin via MBTD1, which recognizes and binds histone H4 trimethylated at 'Lys-20' (H4K20me), and KAT5 that catalyzes acetylation of 'Lys-15' of histone H2A (H2AK15ac), thereby blocking the ubiquitination mark required for TP53BP1 localization at DNA breaks. Also involved in DSB repair by mediating acetylation of 'Lys-5' of histone H2AX (H2AXK5ac), promoting NBN/NBS1 assembly at the sites of DNA damage. 1. Frank, SR. et al. (2003) EMBO reports 4, 575-80. PMID: 12776177 2. Doyon, Y. et al. (2004) Molecular and cellular biology 24, 1884-96. PMID: 14966270 3. Berns, K. et al. (2004) Nature 428, 431-7. PMID: 15042092 4. Taubert, S. et al. (2004) Molecular and cellular biology 24, 4546-56. PMID: 15121871 5. Legube, G. et al. (2004) The Journal of biological chemistry 279, 44825-33. PMID: 15310756 6. Doyon, Y. et al. (2006) Molecular cell 21, 51-64. PMID: 16387653 7. Kikuchi, M. et al. (2009) Biochimica et biophysica acta 1793, 1828-36. PMID: 19909775 8. Cui, H. et al. (2015) Nature communications 6, 6752. PMID: 25865756 9. Jacquet, K. et al. (2016) Molecular cell 62, 409-421. PMID: 27153538 10. Fang, X. et al. (2018) Experimental cell research 362, 279-286. PMID: 29174981 |
| 참고사항 | For research use only. |
SDS-PAGE for Recombinant Human KAT5 Protein, N-His
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