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| 카탈로그 번호 | HC302012 |
|---|---|
| 설명 |
Recombinant Human KEAP1 Protein, N-His (HC302012) expressed in E. coli, spanning Ala90-Ile250. Purity: >90% by SDS-PAGE.
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | Q14145 |
| 단백질 길이 | Ala90-Ile250 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 예측 분자량 | 20.36 kDa |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | INrf2, INRF2, KLHL19, KEAP1, KIAA0132, Kelch-like ECH-associated protein 1, Cytosolic inhibitor of Nrf2, Kelch-like protein 19 |
| 배경 | Kelch-like ECH-associated protein 1 (KEAP1) is a ~69 kDa protein. Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that regulates the response to oxidative stress by targeting NFE2L2/NRF2 for ubiquitination. KEAP1 acts as a key sensor of oxidative and electrophilic stress: in normal conditions, the BCR(KEAP1) complex mediates ubiquitination and degradation of NFE2L2/NRF2, a transcription factor regulating expression of many cytoprotective genes. In response to oxidative stress, different electrophile metabolites trigger non-enzymatic covalent modifications of highly reactive cysteine residues in KEAP1, leading to inactivate the ubiquitin ligase activity of the BCR(KEAP1) complex, promoting NFE2L2/NRF2 nuclear accumulation and expression of phase II detoxifying enzymes. In response to selective autophagy, KEAP1 is sequestered in inclusion bodies following its interaction with SQSTM1/p62, leading to inactivation of the BCR(KEAP1) complex and activation of NFE2L2/NRF2. The BCR(KEAP1) complex also mediates ubiquitination of SQSTM1/p62, increasing SQSTM1/p62 sequestering activity and degradation. 1. Zhang, DD. et al. (2003) Molecular and cellular biology 23, 8137-51. PMID: 14585973 2. Strachan, GD. et al. (2004) Biochemistry 43, 12113-22. PMID: 15379550 3. Zhang, DD. et al. (2004) Molecular and cellular biology 24, 10941-53. PMID: 15572695 4. Furukawa, M. et al. (2005) Molecular and cellular biology 25, 162-71. PMID: 15601839 5. Zhang, DD. et al. (2005) The Journal of biological chemistry 280, 30091-9. PMID: 15983046 6. Cai, C. et al. (2023) Nature communications 14, 3648. PMID: 37339955 7. Eggler, AL. et al. (2005) Proceedings of the National Academy of Sciences of the United States of America 102, 10070-5. PMID: 16006525 8. Gao, L. et al. (2007) The Journal of biological chemistry 282, 2529-37. PMID: 17127771 10. Eggler, AL. et al. (2009) The Biochemical journal 422, 171-80. PMID: 19489739 |
| 참고사항 | For research use only. |
SDS-PAGE for Human KEAP1 Recombinant Protein (N-His)
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