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| 카탈로그 번호 | HC812012 |
|---|---|
| 설명 |
Recombinant Human LAPTM4B Protein, N-GST & C-His (HC812012) expressed in E. coli, spanning Thr2-Thr116. Purity: >90% by SDS-PAGE.
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | Q86VI4 |
| 단백질 길이 | Thr2-Thr116 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 예측 분자량 | 40.65 kDa |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | LAPTM4B, Lysosomal-associated transmembrane protein 4B, Lysosome-associated transmembrane protein 4-beta |
| 배경 | Lysosomal-associated transmembrane protein 4B is a ~35 kDa protein. Required for optimal lysosomal function. Blocks EGF-stimulated EGFR intraluminal sorting and degradation. Conversely by binding with the phosphatidylinositol 4,5-bisphosphate, regulates its PIP5K1C interaction, inhibits HGS ubiquitination and relieves LAPTM4B inhibition of EGFR degradation. Recruits SLC3A2 and SLC7A5 (the Leu transporter) to the lysosome, promoting entry of leucine and other essential amino acid (EAA) into the lysosome, stimulating activation of proton-transporting vacuolar (V)-ATPase protein pump (V-ATPase) and hence mTORC1 activation. Plays a role as negative regulator of TGFB1 production in regulatory T cells. 1. Vergarajauregui, S. et al. (2011) Journal of cell science 124, 459-68. PMID: 21224396 2. Tan, X. et al. (2015) The EMBO journal 34, 475-90. PMID: 25588945 3. Milkereit, R. et al. (2015) Nature communications 6, 7250. PMID: 25998567 4. Huygens, C. et al. (2015) The Journal of biological chemistry 290, 20105-16. PMID: 26126825 5. Blom, T. et al. (2015) Nature chemical biology 11, 799-806. PMID: 26280656 |
| 참고사항 | For research use only |
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