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Human NDUFA4L2 Recombinant Protein (N-His-SUMO & C-Strep) (HP730012)

Human NDUFA4L2 Recombinant Protein (N-His-SUMO & C-Strep)
Human NDUFA4L2 Recombinant Protein (N-His-SUMO & C-Strep)
가격(USD):
사양:
  • 50ug
  • 100ug
  • 1mg
수량:
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  • 개요

  • 이미지

  • 참고 문헌

  • 데이터시트

  • SDS

개요
카탈로그 번호HP730012
설명
Recombinant Human NDUFA4L2 Protein, N-His-SUMO & C-Strep (HP730012) expressed in E. coli, spanning Gln13-Phe87. Purity: >90% by SDS-PAGE.
Highlights
  • ●His-Tagged — N-terminal 6×His tag for IMAC purification.
  • ●E. coli Expression — High-yield, cost-effective production.
  • ●High Purity — >90% purity verified by SDS-PAGE.
발현 시스템E. coli
등록번호Q9NRX3
단백질 길이Gln13-Phe87
응용 분야ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
종Homo sapiens (Human)
성질 Recombinant
내독소 수준 Please contact with the lab for this information.
순도 >90% as determined by SDS-PAGE.
예측 분자량 22.31 kDa
형태 Lyophilized
저장 완충액 Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

재구성 Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.

RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
배송 In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
안정성 및 저장 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
대체 이름NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 4-like 2, NADH-ubiquinone oxidoreductase MLRQ subunit homolog, NDUFA4L2, NUOMS
배경

Cytochrome c oxidase hypoxia associated subunit FA4L2 (NDUFA4L2) is a ~9 kDa protein. Mitochondrial protein that plays a regulatory role in cellular metabolism, particularly under hypoxic conditions. Downregulates mitochondrial respiratory chain complex I activity under hypoxia, reduces oxygen consumption and ROS and helps cells adapt by switching to glycolysis.

1. Tello, D. et al. (2011) Cell metabolism 14, 768-79. PMID: 22100406
참고사항 For research use only.
이미지
  • Human NDUFA4L2 Recombinant Protein (N-His-SUMO & C-Strep)

    SDS-PAGE

    SDS-PAGE for Recombinant Human NDUFA4L2 Protein, N-His-SUMO & C-Strep

참고 문헌
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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