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| 카탈로그 번호 | HV001012 |
|---|---|
| 설명 |
Recombinant Human NLRP3 Protein, N-His (HV001012) expressed in E. coli, spanning Met1-Arg157. Purity: >90% by SDS-PAGE.
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | Q96P20 |
| 단백질 길이 | Met1-Arg157 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 예측 분자량 | 20.82 kDa |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | NLRP3, NACHT, LRR and PYD domains-containing protein 3, Caterpiller protein 1.1, C1orf7, PYPAF1, PYRIN-containing APAF1-like protein 1, CIAS1, CLR1.1, Cold-induced autoinflammatory syndrome 1 protein, Cryopyrin, Angiotensin/vasopressin receptor AII/AVP-like, NALP3 |
| 배경 | NACHT, LRR and PYD domains-containing protein 3 (NLRP3) is a ~118 kDa protein. Sensor component of the NLRP3 inflammasome, which mediates inflammasome activation in response to defects in membrane integrity, leading to secretion of inflammatory cytokines IL1B and IL18 and pyroptosis. In response to pathogens and other damage-associated signals that affect the integrity of membranes, initiates the formation of the inflammasome polymeric complex composed of NLRP3, CASP1 and PYCARD/ASC. Recruitment of pro-caspase-1 (proCASP1) to the NLRP3 inflammasome promotes caspase-1 (CASP1) activation, which subsequently cleaves and activates inflammatory cytokines IL1B and IL18 and gasdermin-D (GSDMD), promoting cytokine secretion and pyroptosis. Activation of NLRP3 inflammasome is also required for HMGB1 secretion; stimulating inflammatory responses. Involved in the homeostatic wound healing response to tissue injury, a multistep cascade that guides neutrophil migration to necrotic sites while avoiding collateral damage of healthy tissues. 1. Martinon, F. et al. (2006) Nature 440, 237-41. PMID: 16407889 2. Dostert, C. et al. (2008) Science (New York, N.Y.) 320, 674-7. PMID: 18403674 3. Hornung, V. et al. (2008) Nature immunology 9, 847-56. PMID: 18604214 4. Subramanian, N. et al. (2013) Cell 153, 348-61. PMID: 23582325 5. Coll, RC. et al. (2015) Nature medicine 21, 248-55. PMID: 25686105 6. Song, H. et al. (2016) Nature communications 7, 13727. PMID: 27929086 7. Li, X. et al. (2017) Nature communications 8, 15986. PMID: 28656979 8. Nakanishi, H. et al. (2017) Proceedings of the National Academy of Sciences of the United States of America 114, E7766-E7775. PMID: 28847925 9. Chen, J. et al. (2018) Nature 564, 71-76. PMID: 30487600 10. Mehto, S. et al. (2019) Molecular cell 73, 429-445.e7. PMID: 30612879 |
| 참고사항 | For research use only. |
SDS-PAGE for Recombinant Human NLRP3 Protein, N-His
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