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| 카탈로그 번호 | HT271011 | ||||||
|---|---|---|---|---|---|---|---|
| 설명 |
Recombinant Human OPA1 Protein, C-His (HT271011) expressed in Mammalian Cells, spanning Gln254-Lys997. Purity: >90% by SDS-PAGE. Highlights
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| 발현 시스템 | Mammalian cells | ||||||
| 등록번호 | O60313 | ||||||
| 단백질 길이 | Gln254-Lys997 | ||||||
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress | ||||||
| 종 | Homo sapiens (Human) | ||||||
| 성질 | Recombinant | ||||||
| 내독소 수준 | Please contact with the lab for this information. | ||||||
| 순도 | >90% as determined by SDS-PAGE. | ||||||
| 예측 분자량 | 89.49 kDa | ||||||
| 형태 | Lyophilized | ||||||
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
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| 재구성 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
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| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. | ||||||
| 제품 사용 정보 |
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| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||
| 대체 이름 | KIAA0567, OPA1, Optic atrophy protein 1, Dynamin-like 120 kDa protein, mitochondrial | ||||||
| 배경 | Dynamin-like GTPase OPA1, mitochondrial is a ~111 kDa protein. Dynamin-related GTPase that is essential for normal mitochondrial morphology by mediating fusion of the mitochondrial inner membranes, regulating cristae morphology and maintaining respiratory chain function. Exists in two forms: the transmembrane, long form (Dynamin-like GTPase OPA1, long form; L-OPA1), which is tethered to the inner mitochondrial membrane, and the short soluble form (Dynamin-like GTPase OPA1, short form; S-OPA1), which results from proteolytic cleavage and localizes in the intermembrane space. Both forms (L-OPA1 and S-OPA1) cooperate to catalyze the fusion of the mitochondrial inner membrane. The equilibrium between L-OPA1 and S-OPA1 is essential: excess levels of S-OPA1, produced by cleavage by OMA1 following loss of mitochondrial membrane potential, lead to an impaired equilibrium between L-OPA1 and S-OPA1, inhibiting mitochondrial fusion. The balance between L-OPA1 and S-OPA1 also influences cristae shape and morphology. 1. Ishihara, N. et al. (2006) The EMBO journal 25, 2966-77. PMID: 16778770 2. Song, Z. et al. (2007) The Journal of cell biology 178, 749-55. PMID: 17709429 3. Ban, T. et al. (2010) Human molecular genetics 19, 2113-22. PMID: 20185555 4. Anand, R. et al. (2014) The Journal of cell biology 204, 919-29. PMID: 24616225 5. Ban, T. et al. (2017) Nature cell biology 19, 856-863. PMID: 28628083 6. Huang, G. et al. (2017) Cell reports 20, 923-934. PMID: 28746876 7. Ge, Y. et al. (2020) eLife 9. PMID: 31922487 8. Zhang, D. et al. (2020) eLife 9. PMID: 32228866 9. Stephan, T. et al. (2020) The EMBO journal 39, e104105. PMID: 32567732 10. Hu, C. et al. (2020) Cell death & disease 11, 940. PMID: 33130824 | ||||||
| 참고사항 | For research use only. |
SDS-PAGE for Human OPA1 Recombinant Protein (C-His)
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