中文
English
한국어
日本語
Español
Français
Русский
| 카탈로그 번호 | HC722012 |
|---|---|
| 설명 |
Recombinant Human ORAI3 Protein, N-His-SUMO (HC722012) expressed in E. coli, spanning Gly178-Gln243. Purity: >90% by SDS-PAGE.
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | Q9BRQ5 |
| 단백질 길이 | Gly178-Gln243 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 예측 분자량 | 18.68 kDa |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | ORAI3, Protein orai-3, TMEM142C, Transmembrane protein 142C |
| 배경 | Protein orai-3 is a ~31 kDa protein. Pore-forming subunit of two major inward rectifying Ca(2+) channels at the plasma membrane: Ca(2+) release-activated Ca(2+) (CRAC) channels and arachidonate-regulated Ca(2+)-selective (ARC) channels. Assembles with ORAI1 and ORAI2 to form hexameric CRAC channels that mediate Ca(2+) influx upon depletion of endoplasmic reticulum Ca(2+) store and channel activation by Ca(2+) sensor STIM1, a process known as store-operated Ca(2+) entry (SOCE). Various pore subunit combinations may account for distinct CRAC channel spatiotemporal and cell-type specific dynamics. ORAI1 mainly contributes to the generation of Ca(2+) plateaus involved in sustained Ca(2+) entry and is dispensable for cytosolic Ca(2+) oscillations, whereas ORAI2 and ORAI3 generate oscillatory patterns. CRAC channels assemble in Ca(2+) signaling microdomains where Ca(2+) influx is coupled to calmodulin and calcineurin signaling and activation of NFAT transcription factors recruited to ORAI1 via AKAP5. 1. Mercer, JC. et al. (2006) The Journal of biological chemistry 281, 24979-90. PMID: 16807233 2. Lis, A. et al. (2007) Current biology : CB 17, 794-800. PMID: 17442569 3. Yuan, JP. et al. (2009) Nature cell biology 11, 337-43. PMID: 19182790 4. Mignen, O. et al. (2009) The Journal of physiology 587, 4181-97. PMID: 19622606 5. Lee, KP. et al. (2009) Proceedings of the National Academy of Sciences of the United States of America 106, 14687-92. PMID: 19706554 6. Bogeski, I. et al. (2010) Science signaling 3, ra24. PMID: 20354224 7. Yoast, RE. et al. (2020) Nature communications 11, 2444. PMID: 32415068 |
| 참고사항 | For research use only |
+86-027-65523339
중국 우한시 심둔사로 666번지 C동, 우한, 430206
