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| 카탈로그 번호 | HV249012 |
|---|---|
| 설명 |
Recombinant Human PARP2 Protein, N-His (HV249012) expressed in E. coli, spanning Ser223-Trp583. Purity: >90% by SDS-PAGE.
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | Q9UGN5 |
| 단백질 길이 | Ser223-Trp583 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 예측 분자량 | 43.19 kDa |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | hPARP-2, PARP-2, ADPRT-2, NAD(+) ADP-ribosyltransferase 2, ADPRTL2, PARP2, ARTD2, Poly[ADP-ribose] synthase 2, Protein poly-ADP-ribosyltransferase PARP2, DNA ADP-ribosyltransferase PARP2, ADPRT2, ADP-ribosyltransferase diphtheria toxin-like 2, Poly [ADP-ribose] polymerase 2, pADPRT-2 |
| 배경 | Poly [ADP-ribose] polymerase 2 (PARP2) is a ~66 kDa protein. Poly-ADP-ribosyltransferase that mediates poly-ADP-ribosylation of proteins and plays a key role in DNA repair. Mediates glutamate, aspartate or serine ADP-ribosylation of proteins: the ADP-D-ribosyl group of NAD(+) is transferred to the acceptor carboxyl group of target residues and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units. Serine ADP-ribosylation of proteins constitutes the primary form of ADP-ribosylation of proteins in response to DNA damage. Mediates glutamate and aspartate ADP-ribosylation of target proteins in absence of HPF1. Following interaction with HPF1, catalyzes serine ADP-ribosylation of target proteins; HPF1 conferring serine specificity by completing the PARP2 active site. 1. Amé, JC. et al. (1999) The Journal of biological chemistry 274, 17860-8. PMID: 10364231 2. Vyas, S. et al. (2014) Nature communications 5, 4426. PMID: 25043379 3. Talhaoui, I. et al. (2016) Nucleic acids research 44, 9279-9295. PMID: 27471034 4. Chen, Q. et al. (2018) Nature communications 9, 3233. PMID: 30104678 5. Suskiewicz, MJ. et al. (2020) Nature 579, 598-602. PMID: 32028527 6. Bilokapic, S. et al. (2020) Nature 585, 609-613. PMID: 32939087 7. Obaji, E. et al. (2021) Nature communications 12, 3479. PMID: 34108479 8. Bacic, L. et al. (2021) eLife 10. PMID: 34486521 9. Mohapatra, J. et al. (2021) eLife 10. PMID: 34874266 |
| 참고사항 | For research use only. |
SDS-PAGE for Recombinant Human PARP2 Protein, N-His
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