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| 카탈로그 번호 | HF611012 |
|---|---|
| 설명 |
Recombinant Human PDCD6 Protein, N-His (HF611012) expressed in E. coli, spanning Phe32-Val191. Purity: >90% by SDS-PAGE.
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | O75340 |
| 단백질 길이 | Phe32-Val191 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 예측 분자량 | 21.09 kDa |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | Apoptosis-linked gene 2 protein homolog, PDCD6, Programmed cell death protein 6, ALG-2, ALG2 |
| 배경 | Programmed cell death protein 6 (PDCD6) is a ~21 kDa protein. Calcium sensor that plays a key role in processes such as endoplasmic reticulum (ER)-Golgi vesicular transport, endosomal biogenesis or membrane repair. Acts as an adapter that bridges unrelated proteins or stabilizes weak protein-protein complexes in response to calcium: calcium-binding triggers exposure of apolar surface, promoting interaction with different sets of proteins thanks to 3 different hydrophobic pockets, leading to translocation to membranes. Involved in ER-Golgi transport by promoting the association between PDCD6IP and TSG101, thereby bridging together the ESCRT-III and ESCRT-I complexes. Together with PEF1, acts as a calcium-dependent adapter for the BCR(KLHL12) complex, a complex involved in ER-Golgi transport by regulating the size of COPII coats. In response to cytosolic calcium increase, the heterodimer formed with PEF1 interacts with, and bridges together the BCR(KLHL12) complex and SEC31 (SEC31A or SEC31B), promoting monoubiquitination of SEC31 and subsequent collagen export, which is required for neural crest specification. 1. Inuzuka, T. et al. (2010) BMC structural biology 10, 25. PMID: 20691033 2. Takahashi, T. et al. (2015) International journal of molecular sciences 16, 3677-99. PMID: 25667979 3. Okumura, M. et al. (2009) Biochemical and biophysical research communications 386, 237-41. PMID: 19520058 4. McGourty, CA. et al. (2016) Cell 167, 525-538.e14. PMID: 27716508 5. Vergarajauregui, S. et al. (2009) The Journal of biological chemistry 284, 36357-36366. PMID: 19864416 6. Kanadome, T. et al. (2017) The FEBS journal 284, 56-76. PMID: 27813252 7. Lee, JH. et al. (2005) Biotechnology letters 27, 1011-5. PMID: 16132846 8. Rho, SB. et al. (2012) Cellular signalling 24, 131-9. PMID: 21893193 9. Ma, J. et al. (2016) The Journal of biological chemistry 291, 26670-26685. PMID: 27784779 |
| 참고사항 | For research use only. |
SDS-PAGE for Recombinant Human PDCD6 Protein, N-His
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