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| 카탈로그 번호 | HA119012 |
|---|---|
| 설명 |
Recombinant Human PRMT1 Protein, N-His (HA119012) expressed in E. coli, spanning Gly61-Arg371. Purity: >90% by SDS-PAGE.
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | Q99873 |
| 단백질 길이 | Gly61-Arg371 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 예측 분자량 | 38.25 kDa |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | IR1B4, Protein arginine N-methyltransferase 1, HRMT1L2, HMT2, Interferon receptor 1-bound protein 4, PRMT1, Histone-arginine N-methyltransferase PRMT1 |
| 배경 | Protein arginine N-methyltransferase 1 (PRMT1) is a ~42 kDa protein. Arginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, FMR1, ILF3, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15, EWS, HABP4, SERBP1, RBM15, FOXO1, CHTOP, MAP3K5/ASK1, MICU1 and NPRL2. Constitutes the main enzyme that mediates monomethylation and asymmetric dimethylation of histone H4 'Arg-3' (H4R3me1 and H4R3me2a, respectively), a specific tag for epigenetic transcriptional activation. May be involved in the regulation of TAF15 transcriptional activity, act as an activator of estrogen receptor (ER)-mediated transactivation, play a key role in neurite outgrowth and act as a negative regulator of megakaryocytic differentiation, by modulating p38 MAPK pathway. Methylates RBM15, promoting ubiquitination and degradation of RBM15. Methylates MRE11 and TP53BP1, promoting the DNA damage response. 1. Tang, J. et al. (2000) The Journal of biological chemistry 275, 19866-76. PMID: 10749851 2. Boisvert, FM. et al. (2005) Genes & development 19, 671-6. PMID: 15741314 3. Passos, DO. et al. (2006) The FEBS journal 273, 3946-61. PMID: 16879614 4. Yamagata, K. et al. (2008) Molecular cell 32, 221-31. PMID: 18951090 5. Cho, JH. et al. (2012) Cell death and differentiation 19, 859-70. PMID: 22095282 6. Takai, H. et al. (2014) Cell reports 9, 48-60. PMID: 25284789 7. Zhang, L. et al. (2015) eLife 4. PMID: 26575292 8. Toma-Fukai, S. et al. (2016) Journal of molecular biology 428, 1197-1208. PMID: 26876602 9. Madreiter-Sokolowski, CT. et al. (2016) Nature communications 7, 12897. PMID: 27642082 10. Tsang, B. et al. (2019) Proceedings of the National Academy of Sciences of the United States of America 116, 4218-4227. PMID: 30765518 |
| 참고사항 | For research use only. |
SDS-PAGE for Human PRMT1 Recombinant Protein (N-His)
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