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| 카탈로그 번호 | HX944012 |
|---|---|
| 설명 |
Recombinant Human PTEN Protein, N-His (HX944012) expressed in E. coli, spanning Met35-Val403. Purity: >90% by SDS-PAGE.
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | P60484 |
| 단백질 길이 | Met35-Val403 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 예측 분자량 | 45.29 kDa |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | Mutated in multiple advanced cancers 1, Phosphatidylinositol 3, 4, 5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN, MMAC1, PTEN, TEP1, Phosphatase and tensin homolog |
| 배경 | Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN is a ~47 kDa protein. Dual-specificity protein phosphatase, dephosphorylating tyrosine-, serine- and threonine-phosphorylated proteins. Also functions as a lipid phosphatase, removing the phosphate in the D3 position of the inositol ring of PtdIns(3,4,5)P3/phosphatidylinositol 3,4,5-trisphosphate, PtdIns(3,4)P2/phosphatidylinositol 3,4-diphosphate and PtdIns3P/phosphatidylinositol 3-phosphate with a preference for PtdIns(3,4,5)P3. Furthermore, this enzyme can also act as a cytosolic inositol 3-phosphatase acting on Ins(1,3,4,5,6)P5/inositol 1,3,4,5,6 pentakisphosphate and possibly Ins(1,3,4,5)P4/1D-myo-inositol 1,3,4,5-tetrakisphosphate. Antagonizes the PI3K-AKT/PKB signaling pathway by dephosphorylating phosphoinositides and thereby modulating cell cycle progression and cell survival. The unphosphorylated form cooperates with MAGI2 to suppress AKT1 activation. 1. Li, DM. et al. (1997) Cancer research 57, 2124-9. PMID: 9187108 2. Myers, MP. et al. (1997) Proceedings of the National Academy of Sciences of the United States of America 94, 9052-7. PMID: 9256433 3. Tamura, M. et al. (1998) Science (New York, N.Y.) 280, 1614-7. PMID: 9616126 4. Vandeput, F. et al. (2006) Cellular signalling 18, 2193-9. PMID: 16824732 5. Costa, HA. et al. (2015) Proceedings of the National Academy of Sciences of the United States of America 112, 13976-81. PMID: 26504226 6. Maehama, T. et al. (1998) The Journal of biological chemistry 273, 13375-8. PMID: 9593664 7. Myers, MP. et al. (1998) Proceedings of the National Academy of Sciences of the United States of America 95, 13513-8. PMID: 9811831 8. Caffrey, JJ. et al. (2001) FEBS letters 499, 6-10. PMID: 11418101 9. Deleu, S. et al. (2006) Cellular signalling 18, 488-98. PMID: 15979280 10. Zhang, J. et al. (2020) Cellular and molecular life sciences : CMLS 77, 2181-2197. PMID: 31492966 |
| 참고사항 | For research use only. |
SDS-PAGE for Recombinant Human PTEN Protein, N-His
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