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| 카탈로그 번호 | HB244012 |
|---|---|
| 설명 |
Recombinant Human RAB5A Protein, N-His (HB244012) expressed in E. coli, spanning Asp136-Asn215. Purity: >90% by SDS-PAGE.
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | P20339 |
| 단백질 길이 | Asp136-Asn215 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 예측 분자량 | 11.08 kDa |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | Ras-related protein Rab-5A, RAB5A, RAB5 |
| 배경 | Ras-related protein Rab-5A (RAB5A) is a ~23 kDa protein. The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes and involved in early endocytic trafficking. Required for EEA1 recruitment to early endosomes. Recruits FERRY complex to early endosomes, where FERRY links early endosomes with a subgroup of mRNAs to enable mRNA intracellular distribution. 1. Hoffenberg, S. et al. (2000) The Journal of biological chemistry 275, 24661-9. PMID: 10818110 2. Fouraux, MA. et al. (2004) Molecular biology of the cell 15, 611-24. PMID: 14617813 3. Zhu, G. et al. (2004) Nature structural & molecular biology 11, 975-83. PMID: 15378032 4. Haas, AK. et al. (2005) Nature cell biology 7, 887-93. PMID: 16086013 5. Hunker, CM. et al. (2006) Biochemical and biophysical research communications 340, 967-75. PMID: 16410077 6. Fuchs, E. et al. (2007) The Journal of cell biology 177, 1133-43. PMID: 17562788 7. Quentin, D. et al. (2023) Molecular cell 83, 1856-1871.e9. PMID: 37267906 8. Gauthier-Campbell, C. et al. (2004) Molecular biology of the cell 15, 2205-17. PMID: 14978216 9. Baietti, MF. et al. (2012) Nature cell biology 14, 677-85. PMID: 22660413 |
| 참고사항 | For research use only. |
SDS-PAGE for Recombinant Human RAB5A Protein, N-His
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