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| 카탈로그 번호 | HC317012 |
|---|---|
| 설명 |
Recombinant Human RIPK1 Protein, N-His (HC317012) expressed in E. coli, spanning Met1-Asn671. Purity: >90% by SDS-PAGE.
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | Q13546 |
| 단백질 길이 | Met1-Asn671 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 예측 분자량 | 78.09 kDa |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | Receptor-interacting protein 1, RIP1, RIPK1, RIP, Cell death protein RIP, RIP-1, Receptor-interacting serine/threonine-protein kinase 1 |
| 배경 | Receptor-interacting serine/threonine-protein kinase 1 (RIPK1) is a ~75 kDa protein. Serine-threonine kinase which is a key regulator of TNF-mediated apoptosis, necroptosis and inflammatory pathways. Exhibits kinase activity-dependent functions that regulate cell death and kinase-independent scaffold functions regulating inflammatory signaling and cell survival. Has kinase-independent scaffold functions: upon binding of TNF to TNFR1, RIPK1 is recruited to the TNF-R1 signaling complex (TNF-RSC also known as complex I) where it acts as a scaffold protein promoting cell survival, in part, by activating the canonical NF-kappa-B pathway. Kinase activity is essential to regulate necroptosis and apoptosis, two parallel forms of cell death: upon activation of its protein kinase activity, regulates assembly of two death-inducing complexes, namely complex IIa (RIPK1-FADD-CASP8), which drives apoptosis, and the complex IIb (RIPK1-RIPK3-MLKL), which drives necroptosis. RIPK1 is required to limit CASP8-dependent TNFR1-induced apoptosis. 1. Shembade, N. et al. (2007) The EMBO journal 26, 3910-22. PMID: 17703191 2. Zaman, MM. et al. (2013) Molecular and cellular biology 33, 4971-84. PMID: 24144979 3. Tao, P. et al. (2020) Nature 577, 109-114. PMID: 31827280 4. Lalaoui, N. et al. (2020) Nature 577, 103-108. PMID: 31827281 5. Ashida, H. et al. (2020) The EMBO journal 39, e104469. PMID: 32657447 6. Hou, B. et al. (2022) Cell death discovery 8, 319. PMID: 35831301 7. Holler, N. et al. (2000) Nature immunology 1, 489-95. PMID: 11101870 8. He, S. et al. (2009) Cell 137, 1100-11. PMID: 19524512 9. Cho, YS. et al. (2009) Cell 137, 1112-23. PMID: 19524513 10. Meng, H. et al. (2018) Proceedings of the National Academy of Sciences of the United States of America 115, E2001-E2009. PMID: 29440439 |
| 참고사항 | For research use only. |
SDS-PAGE for Recombinant Human RIPK1 Protein, N-His
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