Human RIPK3 Recombinant Protein (N-GST & C-His) (HV460022)

Name: Human RIPK3 Recombinant Protein (N-GST & C-His)
Brand: abinScience
SKU: HV460022
Price: 211 USD
Availability: InStock

데이터시트 제품 공유 정보 복사

가격(USD):

사양:

50ug
100ug
1mg

수량:

문의하기

개요
이미지
참고 문헌
데이터시트
SDS

개요

카탈로그 번호	HV460022
설명	Recombinant Human RIPK3 Protein, N-GST & C-His (HV460022) expressed in E. coli, spanning Met1-His132. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
발현 시스템	E. coli
등록번호	Q9Y572
단백질 길이	Met1-His132
응용 분야	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
종	Homo sapiens (Human)
성질	Recombinant
내독소 수준	Please contact with the lab for this information.
순도	>90% as determined by SDS-PAGE.
예측 분자량	42.96 kDa
형태	Lyophilized
저장 완충액	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.
재구성	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
배송	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
안정성 및 저장	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
대체 이름	EC:2.7.11.1, RIP-3, RIP-like protein kinase 3, RIP3, RIPK3, Receptor-interacting protein 3, Receptor-interacting serine/threonine-protein kinase 3
배경	Receptor-interacting serine/threonine-protein kinase 3 is a ~56 kDa protein. Serine/threonine-protein kinase that activates necroptosis and apoptosis, two parallel forms of cell death. Necroptosis, a programmed cell death process in response to death-inducing TNF family members, is triggered by RIPK3 following activation by ZBP1. Activated RIPK3 forms a necrosis-inducing complex and mediates phosphorylation of MLKL, promoting MLKL localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. In addition to TNF-induced necroptosis, necroptosis can also take place in the nucleus in response to orthomyxoviruses infection: following ZBP1 activation, which senses double-stranded Z-RNA structures, nuclear RIPK3 catalyzes phosphorylation and activation of MLKL, promoting disruption of the nuclear envelope and leakage of cellular DNA into the cytosol. Also regulates apoptosis: apoptosis depends on RIPK1, FADD and CASP8, and is independent of MLKL and RIPK3 kinase activity. 1. He, S. et al. (2009) Cell 137, 1100-11. PMID: 19524512 2. Cho, YS. et al. (2009) Cell 137, 1112-23. PMID: 19524513 3. Sun, L. et al. (2012) Cell 148, 213-27. PMID: 22265413 4. Wang, Z. et al. (2012) Cell 148, 228-43. PMID: 22265414 5. Zhao, J. et al. (2012) Proceedings of the National Academy of Sciences of the United States of America 109, 5322-7. PMID: 22421439 6. Choi, SW. et al. (2018) Molecular cell 70, 920-935.e7. PMID: 29883609 7. Ashida, H. et al. (2020) The EMBO journal 39, e104469. PMID: 32657447 8. Zheng, M. et al. (2020) Cell 181, 674-687.e13. PMID: 32298652
참고사항	For research use only