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| 카탈로그 번호 | HD914012 |
|---|---|
| 설명 |
Recombinant Human SHARPIN Protein, N-His (HD914012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | Q9H0F6 |
| 단백질 길이 | Ala18-Gly126 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | SHARPIN, SIPL1, Shank-associated RH domain-interacting protein, Shank-interacting protein-like 1, Sharpin, hSIPL1 |
| 배경 | Sharpin is a ~39 kDa protein. Component of the LUBAC complex which conjugates linear polyubiquitin chains in a head-to-tail manner to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. The LUBAC complex is also involved in innate immunity by conjugating linear polyubiquitin chains at the surface of bacteria invading the cytosol to form the ubiquitin coat surrounding bacteria. 1. Gerlach, B. et al. (2011) Nature 471, 591-6. PMID: 21455173 2. Tokunaga, F. et al. (2011) Nature 471, 633-6. PMID: 21455180 3. Ikeda, F. et al. (2011) Nature 471, 637-41. PMID: 21455181 4. Noad, J. et al. (2017) Nature microbiology 2, 17063. PMID: 28481331 |
| 참고사항 | For research use only |
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