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| 카탈로그 번호 | HD381012 |
|---|---|
| 설명 |
Recombinant Human SIAH1 Protein, N-His (HD381012) expressed in E. coli, spanning Thr29-Cys282. Purity: >90% by SDS-PAGE.
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | Q8IUQ4 |
| 단백질 길이 | Thr29-Cys282 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 예측 분자량 | 30.58 kDa |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | E3 ubiquitin-protein ligase SIAH1, EC:2.3.2.27, HUMSIAH, RING-type E3 ubiquitin transferase SIAH1, SIAH1, Seven in absentia homolog 1, Siah-1, Siah-1a |
| 배경 | E3 ubiquitin-protein ligase SIAH1 is a ~31 kDa protein. E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates E3 ubiquitin ligase activity either through direct binding to substrates or by functioning as the essential RING domain subunit of larger E3 complexes. Triggers the ubiquitin-mediated degradation of many substrates, including proteins involved in transcription regulation (ELL2, MYB, POU2AF1, PML and RBBP8), a cell surface receptor (DCC), the cell-surface receptor-type tyrosine kinase FLT3, the cytoplasmic signal transduction molecules (KLF10/TIEG1 and NUMB), an antiapoptotic protein (BAG1), a microtubule motor protein (KIF22), a protein involved in synaptic vesicle function in neurons (SYP), a structural protein (CTNNB1) and SNCAIP. Confers constitutive instability to HIPK2 through proteasomal degradation. 1. Nagano, Y. et al. (2003) The Journal of biological chemistry 278, 51504-14. PMID: 14506261 2. Fanelli, M. et al. (2004) The Journal of biological chemistry 279, 5374-9. PMID: 14645235 3. Germani, A. et al. (2003) Oncogene 22, 8845-51. PMID: 14654780 4. Liani, E. et al. (2004) Proceedings of the National Academy of Sciences of the United States of America 101, 5500-5. PMID: 15064394 5. Santelli, E. et al. (2005) The Journal of biological chemistry 280, 34278-87. PMID: 16085652 6. Szargel, R. et al. (2009) The Journal of biological chemistry 284, 11706-16. PMID: 19224863 7. Buchwald, M. et al. (2010) Leukemia 24, 1412-21. PMID: 20508617 8. Liu, M. et al. (2012) Molecular cell 46, 325-34. PMID: 22483617 9. Ji, L. et al. (2017) Genes & development 31, 904-915. PMID: 28546513 10. Buratti, J. et al. (2021) Journal of medical genetics 58, 205-212. PMID: 32430360 |
| 참고사항 | For research use only |
SDS-PAGE for Human SIAH1 Recombinant Protein (N-His)
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