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Human SLC1A2 Recombinant Protein (N-GST & C-His) (HW629022)

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사양:
  • 50ug
  • 100ug
  • 1mg
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  • 개요

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  • 데이터시트

  • SDS

개요
카탈로그 번호HW629022
설명
Recombinant Human SLC1A2 Protein, N-GST & C-His (HW629022) expressed in E. coli, spanning His143-Glu235. Purity: >90% by SDS-PAGE.
Highlights
  • ●His-Tagged — N-terminal 6×His tag for IMAC purification.
  • ●E. coli Expression — High-yield, cost-effective production.
  • ●High Purity — >90% purity verified by SDS-PAGE.
발현 시스템E. coli
등록번호P43004
단백질 길이His143-Glu235
응용 분야ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
종Homo sapiens (Human)
성질 Recombinant
내독소 수준 Please contact with the lab for this information.
순도 >90% as determined by SDS-PAGE.
예측 분자량 38.36 kDa
형태 Lyophilized
저장 완충액 Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

재구성 Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
배송 In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
안정성 및 저장 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
대체 이름EAAT2, Excitatory amino acid transporter 2, GLT1, Glutamate/aspartate transporter II, SLC1A2, Sodium-dependent glutamate/aspartate transporter 2, Solute carrier family 1 member 2
배경

Excitatory amino acid transporter 2 is a ~62 kDa protein. Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate. Functions as a symporter that transports one amino acid molecule together with two or three Na(+) ions and one proton, in parallel with the counter-transport of one K(+) ion. Mediates Cl(-) flux that is not coupled to amino acid transport; this avoids the accumulation of negative charges due to aspartate and Na(+) symport. Essential for the rapid removal of released glutamate from the synaptic cleft, and for terminating the postsynaptic action of glutamate.

1. Melzer, N. et al. (2003) The Journal of biological chemistry 278, 50112-9. PMID: 14506254
2. Gendreau, S. et al. (2004) The Journal of biological chemistry 279, 39505-12. PMID: 15265858
3. Abousaab, A. et al. (2016) The Journal of membrane biology 249, 239-49. PMID: 26690923
4. Arriza, JL. et al. (1994) The Journal of neuroscience : the official journal of the Society for Neuroscience 14, 5559-69. PMID: 7521911
참고사항 For research use only
이미지
참고 문헌
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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