Human SLC4A7 Recombinant Protein (C-His) (HA125032)

Name: Human SLC4A7 Recombinant Protein (C-His)
Brand: abinScience
SKU: HA125032
Price: 211 USD
Availability: InStock

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50ug
100ug
1mg

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개요
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참고 문헌
데이터시트
SDS

개요

카탈로그 번호	HA125032
설명	Recombinant Human SLC4A7 Protein, C-His (HA125032) expressed in E. coli, spanning Ala1114-Leu1214. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
발현 시스템	E. coli
등록번호	Q9Y6M7
단백질 길이	Ala1114-Leu1214
응용 분야	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
종	Homo sapiens (Human)
성질	Recombinant
내독소 수준	Please contact with the lab for this information.
순도	>90% as determined by SDS-PAGE.
예측 분자량	12.98 kDa
형태	Lyophilized
저장 완충액	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.
재구성	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
배송	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
안정성 및 저장	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
대체 이름	Sodium bicarbonate cotransporter 3; Electroneutral Na/HCO(3) cotransporter; Sodium bicarbonate cotransporter 2; Sodium bicarbonate cotransporter 2b; Bicarbonate transporter; Solute carrier family 4 member 7; SLC4A7; BT; NBC2; NBC2B; NBC3; NBCn1; SBC2; SLC4A6
배경	Sodium bicarbonate cotransporter 3 is a ~136 kDa protein. Electroneutral sodium- and bicarbonate-dependent cotransporter with a Na(+):HCO3(-) 1:1 stoichiometry. Mediates the sodium-dependent bicarbonate transport important for pH recovery after acid load as well as for regulation of steady-state pH in the duodenum and vascular smooth muscle cells. Plays a key role in macrophage acidification, mediating bicarbonate import into the cytoplasm which is crucial for net acid extrusion and maintenance of cytoplasmic pH during phagocytosis. Provides cellular bicarbonate for de novo purine and pyrimidine synthesis and is a key mediator of de novo nucleotide synthesis downstream of mTORC1 signaling in proliferating cells. 1. Pushkin, A. et al. (1999) The Journal of biological chemistry 274, 16569-75. PMID: 10347222 2. Park, M. et al. (2002) The Journal of biological chemistry 277, 50503-9. PMID: 12403779 3. Loiselle, FB. et al. (2003) Molecular membrane biology 20, 307-17. PMID: 14578046 4. Loiselle, FB. et al. (2004) American journal of physiology. Cell physiology 286, C1423-33. PMID: 14736710 5. Sedlyarov, V. et al. (2018) Cell host & microbe 23, 766-774.e5. PMID: 29779931 6. Ali, ES. et al. (2022) Molecular cell 82, 3284-3298.e7. PMID: 35772404
참고사항	For research use only