Human SUPT5H Recombinant Protein (N-His) (HC862012)

Name: Human SUPT5H Recombinant Protein (N-His)
Brand: abinScience
SKU: HC862012
Price: 211 USD
Availability: InStock

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50ug
100ug
1mg

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개요

카탈로그 번호	HC862012
설명	Recombinant Human SUPT5H Protein, N-His (HC862012) expressed in E. coli, spanning Lys274-Val644. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
발현 시스템	E. coli
등록번호	O00267
단백질 길이	Lys274-Val644
응용 분야	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
종	Homo sapiens (Human)
성질	Recombinant
내독소 수준	Please contact with the lab for this information.
순도	>90% as determined by SDS-PAGE.
예측 분자량	44.75 kDa
형태	Lyophilized
저장 완충액	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.
재구성	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
배송	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
안정성 및 저장	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
대체 이름	DRB sensitivity-inducing factor 160 kDa subunit, DRB sensitivity-inducing factor large subunit, DSIF large subunit, DSIF p160, SPT5, SPT5H, SUPT5H, Tat-CT1 protein, Tat-cotransactivator 1 protein, Transcription elongation factor SPT5, hSPT5
배경	Transcription elongation factor SPT5 is a ~121 kDa protein. Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. 1. Yamaguchi, Y. et al. (1999) The Journal of biological chemistry 274, 8085-92. PMID: 10075709 2. Yamaguchi, Y. et al. (1999) Cell 97, 41-51. PMID: 10199401 3. Wen, Y. et al. (1999) Genes & development 13, 1774-9. PMID: 10421630 4. Ivanov, D. et al. (2000) Molecular and cellular biology 20, 2970-83. PMID: 10757782 5. Wada, T. et al. (2000) Molecular cell 5, 1067-72. PMID: 10912001 6. Ping, YH. et al. (2001) The Journal of biological chemistry 276, 12951-8. PMID: 11112772 7. Renner, DB. et al. (2001) The Journal of biological chemistry 276, 42601-9. PMID: 11553615 8. Kim, DK. et al. (2003) Genes to cells : devoted to molecular & cellular mechanisms 8, 371-8. PMID: 12653964 9. Kwak, YT. et al. (2003) Molecular cell 11, 1055-66. PMID: 12718890 10. Mandal, SS. et al. (2004) Proceedings of the National Academy of Sciences of the United States of America 101, 7572-7. PMID: 15136722
참고사항	For research use only

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Human SUPT5H Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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Human SUPT5H Recombinant Protein (N-His) (HC862012)

개요

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참고 문헌

데이터시트

SDS

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