中文
English
한국어
日本語
Español
Français
Русский
| 카탈로그 번호 | HV178012 |
|---|---|
| 설명 |
Recombinant Human TBK1 Protein, N-His (HV178012) expressed in E. coli, spanning Met1-Gly121. Purity: >90% by SDS-PAGE.
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | Q9UHD2 |
| 단백질 길이 | Met1-Gly121 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 예측 분자량 | 15.96 kDa |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | Serine/threonine-protein kinase TBK1, NAK, TANK-binding kinase 1, T2K, TBK1, NF-kappa-B-activating kinase |
| 배경 | Serine/threonine-protein kinase TBK1 is a ~83 kDa protein. Serine/threonine kinase that plays an essential role in regulating inflammatory responses to foreign agents. Following activation of toll-like receptors by viral or bacterial components, associates with TRAF3 and TANK and phosphorylates interferon regulatory factors (IRFs) IRF3 and IRF7 as well as DDX3X. This activity allows subsequent homodimerization and nuclear translocation of the IRFs leading to transcriptional activation of pro-inflammatory and antiviral genes including IFNA and IFNB. In order to establish such an antiviral state, TBK1 form several different complexes whose composition depends on the type of cell and cellular stimuli. Plays a key role in IRF3 activation: acts by first phosphorylating innate adapter proteins MAVS, STING1 and TICAM1 on their pLxIS motif, leading to recruitment of IRF3, thereby licensing IRF3 for phosphorylation by TBK1. 1. Pomerantz, JL. et al. (1999) The EMBO journal 18, 6694-704. PMID: 10581243 2. Kishore, N. et al. (2002) The Journal of biological chemistry 277, 13840-7. PMID: 11839743 3. Fitzgerald, KA. et al. (2003) Nature immunology 4, 491-6. PMID: 12692549 4. Sharma, S. et al. (2003) Science (New York, N.Y.) 300, 1148-51. PMID: 12702806 5. Mori, M. et al. (2004) The Journal of biological chemistry 279, 9698-702. PMID: 14703513 6. tenOever, BR. et al. (2004) Journal of virology 78, 10636-49. PMID: 15367631 7. Kuai, J. et al. (2004) The Journal of biological chemistry 279, 53266-71. PMID: 15485837 8. Soulat, D. et al. (2008) The EMBO journal 27, 2135-46. PMID: 18583960 9. Clark, K. et al. (2011) The Biochemical journal 434, 93-104. PMID: 21138416 10. Larabi, A. et al. (2013) Cell reports 3, 734-46. PMID: 23453971 |
| 참고사항 | For research use only. |
SDS-PAGE for Recombinant Human TBK1 Protein, N-His
+86-027-65523339
중국 우한시 심둔사로 666번지 C동, 우한, 430206
