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| 카탈로그 번호 | HA404012 |
|---|---|
| 설명 |
Recombinant Human TBX21 Protein, C-His (HA404012) expressed in E. coli, spanning Met11-Asn535. Purity: >90% by SDS-PAGE.
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | Q9UL17 |
| 단백질 길이 | Met11-Asn535 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 예측 분자량 | 58.70 kDa |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | T-box transcription factor TBX21, T-box protein 21, T-cell-specific T-box transcription factor T-bet, Transcription factor TBLYM, TBX21, TBLYM, TBET |
| 배경 | T-box transcription factor TBX21 is a ~58 kDa protein. Lineage-defining transcription factor which initiates Th1 lineage development from naive Th precursor cells both by activating Th1 genetic programs and by repressing the opposing Th2 and Th17 genetic programs. Activates transcription of a set of genes important for Th1 cell function, including those encoding IFN-gamma and the chemokine receptor CXCR3. Induces permissive chromatin accessibilty and CpG methylation in IFNG. Activates IFNG and CXCR3 genes in part by recruiting chromatin remodeling complexes including KDM6B, a SMARCA4-containing SWI/SNF-complex, and an H3K4me2-methyltransferase complex to their promoters and all of these complexes serve to establish a more permissive chromatin state conducive with transcriptional activation. Can activate Th1 genes also via recruitment of Mediator complex and P-TEFb (composed of CDK9 and CCNT1/cyclin-T1) in the form of the super elongation complex (SEC) to super-enhancers and associated genes in activated Th1 cells. 1. Szabo, SJ. et al. (2000) Cell 100, 655-69. PMID: 10761931 2. Yang, R. et al. (2020) Cell 183, 1826-1847.e31. PMID: 33296702 3. Hertweck, A. et al. (2016) Cell reports 15, 2756-70. PMID: 27292648 |
| 참고사항 | For research use only. |
SDS-PAGE for Recombinant Human TBX21 Protein, C-His
+86-027-65523339
중국 우한시 심둔사로 666번지 C동, 우한, 430206
