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| 카탈로그 번호 | HS853012 |
|---|---|
| 설명 |
Recombinant Human TERF2 Protein, N-His (HS853012) expressed in E. coli, spanning Arg144-Gln438. Purity: >90% by SDS-PAGE.
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | Q15554 |
| 단백질 길이 | Arg144-Gln438 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 예측 분자량 | 34.85 kDa |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | Telomeric repeat-binding factor 2, Telomeric DNA-binding protein, TERF2, TRBF2, TTAGGG repeat-binding factor 2, TRF2 |
| 배경 | Telomeric repeat-binding factor 2 (TERF2) is a ~59 kDa protein. Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes. In addition to its telomeric DNA-binding role, required to recruit a number of factors and enzymes required for telomere protection, including the shelterin complex, TERF2IP/RAP1 and DCLRE1B/Apollo. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Together with DCLRE1B/Apollo, plays a key role in telomeric loop (T loop) formation by generating 3' single-stranded overhang at the leading end telomeres: T loops have been proposed to protect chromosome ends from degradation and repair. 1. Court, R. et al. (2005) EMBO reports 6, 39-45. PMID: 15608617 2. de Lange, T. (2005) Genes & development 19, 2100-10. PMID: 16166375 3. Ye, J. et al. (2010) Cell 142, 230-42. PMID: 20655466 4. Rai, R. et al. (2017) Molecular cell 65, 801-817.e4. PMID: 28216226 5. Li, P. et al. (2019) International journal of biological sciences 15, 2350-2362. PMID: 31595153 6. Broccoli, D. et al. (1997) Nature genetics 17, 231-5. PMID: 9326950 7. Bilaud, T. et al. (1997) Nature genetics 17, 236-9. PMID: 9326951 8. van Steensel, B. et al. (1998) Cell 92, 401-13. PMID: 9476899 |
| 참고사항 | For research use only. |
SDS-PAGE for Recombinant Human TERF2 Protein, N-His
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