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| 카탈로그 번호 | HB821012 |
|---|---|
| 설명 |
Recombinant Human TFEB Protein, N-His (HB821012) expressed in E. coli, spanning Met1-Leu476. Purity: >90% by SDS-PAGE.
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | P19484 |
| 단백질 길이 | Met1-Leu476 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 예측 분자량 | 55.02 kDa |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | TFEB, Class E basic helix-loop-helix protein 35, BHLHE35, bHLHe35, Transcription factor EB |
| 배경 | Transcription factor EB (TFEB) is a ~52 kDa protein. Transcription factor that acts as a master regulator of lysosomal biogenesis, autophagy, lysosomal exocytosis, lipid catabolism, energy metabolism and immune response. Specifically recognizes and binds E-box sequences (5'-CANNTG-3'); efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. Involved in the cellular response to amino acid availability by acting downstream of MTOR: in the presence of nutrients, TFEB phosphorylation by MTOR promotes its cytosolic retention and subsequent inactivation. Upon starvation or lysosomal stress, inhibition of MTOR induces TFEB dephosphorylation, resulting in nuclear localization and transcription factor activity. Specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression, thereby playing a central role in expression of lysosomal genes. 1. Settembre, C. et al. (2011) Science (New York, N.Y.) 332, 1429-33. PMID: 21617040 2. Settembre, C. et al. (2012) The EMBO journal 31, 1095-108. PMID: 22343943 3. Martina, JA. et al. (2012) Autophagy 8, 903-14. PMID: 22576015 4. Roczniak-Ferguson, A. et al. (2012) Science signaling 5, ra42. PMID: 22692423 5. Medina, DL. et al. (2015) Nature cell biology 17, 288-99. PMID: 25720963 6. Napolitano, G. et al. (2018) Nature communications 9, 3312. PMID: 30120233 7. Lawrence, RE. et al. (2019) Science (New York, N.Y.) 366, 971-977. PMID: 31672913 8. Napolitano, G. et al. (2020) Nature 585, 597-602. PMID: 32612235 9. Kumar, S. et al. (2020) Nature cell biology 22, 973-985. PMID: 32753672 10. Zhang, Z. et al. (2022) Molecular cell 82, 2844-2857.e10. PMID: 35662396 |
| 참고사항 | For research use only. |
SDS-PAGE for Recombinant Human TFEB Protein, N-His
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