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| 카탈로그 번호 | HD758012 |
|---|---|
| 설명 |
Recombinant Human UBA5 Protein, N-His (HD758012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | Q9GZZ9 |
| 단백질 길이 | Lys70-Asn286 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | ThiFP1, UBA5, UBE1DC1, UFM1-activating enzyme, Ubiquitin-activating enzyme 5, Ubiquitin-activating enzyme E1 domain-containing protein 1, Ubiquitin-like modifier-activating enzyme 5 |
| 배경 | Ubiquitin-like modifier-activating enzyme 5 is a ~44 kDa protein. E1-like enzyme which specifically catalyzes the first step in ufmylation. Activates UFM1 by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a UFM1-E1 thioester and free AMP. Activates UFM1 via a trans-binding mechanism, in which UFM1 interacts with distinct sites in both subunits of the UBA5 homodimer. Trans-binding also promotes stabilization of the UBA5 homodimer, and enhances ATP-binding. Transfer of UFM1 from UBA5 to the E2-like enzyme UFC1 also takes place using a trans mechanism. 1. Komatsu, M. et al. (2004) The EMBO journal 23, 1977-86. PMID: 15071506 2. Zheng, M. et al. (2008) Journal of cellular biochemistry 104, 2324-34. PMID: 18442052 3. Bacik, JP. et al. (2010) The Journal of biological chemistry 285, 20273-80. PMID: 20368332 4. Yoo, HM. et al. (2014) Molecular cell 56, 261-274. PMID: 25219498 5. Habisov, S. et al. (2016) The Journal of biological chemistry 291, 9025-41. PMID: 26929408 6. Muona, M. et al. (2016) American journal of human genetics 99, 683-694. PMID: 27545674 7. Colin, E. et al. (2016) American journal of human genetics 99, 695-703. PMID: 27545681 8. Oweis, W. et al. (2016) Cell reports 16, 3113-3120. PMID: 27653677 9. Soudah, N. et al. (2019) Journal of molecular biology 431, 463-478. PMID: 30412706 10. Walczak, CP. et al. (2019) Proceedings of the National Academy of Sciences of the United States of America 116, 1299-1308. PMID: 30626644 |
| 참고사항 | For research use only |
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