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| 카탈로그 번호 | HC286012 |
|---|---|
| 설명 |
Recombinant Human UBE3A Protein, N-His (HC286012) expressed in E. coli, spanning Met291-Leu407. Purity: >90% by SDS-PAGE.
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | Q05086 |
| 단백질 길이 | Met291-Leu407 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 예측 분자량 | 15.61 kDa |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | Oncogenic protein-associated protein E6-AP, Ubiquitin-protein ligase E3A, UBE3A, E6AP ubiquitin-protein ligase, HPVE6A, EPVE6AP, HECT-type ubiquitin transferase E3A, E6AP, Human papillomavirus E6-associated protein, Renal carcinoma antigen NY-REN-54 |
| 배경 | Ubiquitin-protein ligase E3A (UBE3A) is a ~100 kDa protein. E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates. Several substrates have been identified including the BMAL1, ARC, LAMTOR1, RAD23A and RAD23B, MCM7 (which is involved in DNA replication), annexin A1, the PML tumor suppressor, and the cell cycle regulator CDKN1B. Additionally, may function as a cellular quality control ubiquitin ligase by helping the degradation of the cytoplasmic misfolded proteins. Finally, UBE3A also promotes its own degradation in vivo. Plays an important role in the regulation of the circadian clock: involved in the ubiquitination of the core clock component BMAL1, leading to its proteasomal degradation. 1. Kumar, S. et al. (1999) The Journal of biological chemistry 274, 18785-92. PMID: 10373495 2. Dhananjayan, SC. et al. (2006) Molecular endocrinology (Baltimore, Md.) 20, 2343-54. PMID: 16772533 3. Shimoji, T. et al. (2009) Journal of cellular biochemistry 106, 1123-35. PMID: 19204938 4. Mishra, A. et al. (2009) The Journal of biological chemistry 284, 10537-45. PMID: 19233847 5. Louria-Hayon, I. et al. (2009) Cell death and differentiation 16, 1156-66. PMID: 19325566 6. Mishra, A. et al. (2009) Neurobiology of disease 36, 26-34. PMID: 19591933 7. Martínez-Noël, G. et al. (2012) Molecular and cellular biology 32, 3095-106. PMID: 22645313 8. Ronchi, VP. et al. (2014) The Journal of biological chemistry 289, 1033-48. PMID: 24273172 9. Gossan, NC. et al. (2014) Nucleic acids research 42, 5765-75. PMID: 24728990 10. Sun, J. et al. (2018) eLife 7. PMID: 30020076 |
| 참고사항 | For research use only. |
SDS-PAGE for Recombinant Human UBE3A Protein, N-His
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