中文
English
한국어
日本語
Español
Français
Русский
| 카탈로그 번호 | HP604012 |
|---|---|
| 설명 |
Recombinant Human USP13 Protein, N-His (HP604012) expressed in E. coli, spanning Ser344-Ser863. Purity: >90% by SDS-PAGE.
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | Q92995 |
| 단백질 길이 | Ser344-Ser863 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 예측 분자량 | 61.22 kDa |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | Isopeptidase T-3, Deubiquitinating enzyme 13, ISOT-3, ISOT3, Ubiquitin-specific-processing protease 13, Ubiquitin carboxyl-terminal hydrolase 13, Ubiquitin thioesterase 13, USP13 |
| 배경 | Ubiquitin carboxyl-terminal hydrolase 13 (USP13) is a ~97 kDa protein. Deubiquitinase that mediates deubiquitination of target proteins such as BECN1, MITF, SKP2 and USP10 and is involved in various processes such as autophagy, endoplasmic reticulum-associated degradation (ERAD), cell cycle progression or DNA damage response. Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. Alternatively, forms with NEDD4 a deubiquitination complex, which subsequently stabilizes VPS34 to promote autophagy. Also deubiquitinates USP10, an essential regulator of p53/TP53 stability. In turn, PIK3C3/VPS34-containing complexes regulate USP13 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. 1. Chen, M. et al. (2011) Proceedings of the National Academy of Sciences of the United States of America 108, 9119-24. PMID: 21571647 2. Esposito, M. et al. (2020) Oncogene 39, 6009-6023. PMID: 32772043 3. Kim, W. et al. (2021) DNA repair 100, 103063. PMID: 33592542 4. Xie, W. et al. (2020) Cell reports 30, 2807-2819.e4. PMID: 32101753 5. Shin, J. et al. (2022) Molecular medicine (Cambridge, Mass.) 28, 164. PMID: 36585612 6. Yeh, HM. et al. (2013) Journal of immunology (Baltimore, Md. : 1950) 191, 3328-36. PMID: 23940278 7. Sun, H. et al. (2017) Nature communications 8, 15534. PMID: 28534493 |
| 참고사항 | For research use only. |
SDS-PAGE for Recombinant Human USP13 Protein, N-His
+86-027-65523339
중국 우한시 심둔사로 666번지 C동, 우한, 430206
