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Human USP9X Recombinant Protein (N-His) (HC171012)

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사양:
  • 50ug
  • 100ug
  • 1mg
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  • 개요

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개요
카탈로그 번호HC171012
설명
Recombinant Human USP9X Protein, N-His (HC171012) expressed in E. coli, spanning Arg1551-Ser1970. Purity: >90% by SDS-PAGE.
Highlights
  • ●His-Tagged — N-terminal 6×His tag for IMAC purification.
  • ●E. coli Expression — High-yield, cost-effective production.
  • ●High Purity — >90% purity verified by SDS-PAGE.
발현 시스템E. coli
등록번호Q93008
단백질 길이Arg1551-Ser1970
응용 분야ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
종Homo sapiens (Human)
성질 Recombinant
내독소 수준 Please contact with the lab for this information.
순도 >90% as determined by SDS-PAGE.
예측 분자량 50.85 kDa
형태 Lyophilized
저장 완충액 Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

재구성 Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
배송 In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
안정성 및 저장 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
대체 이름DFFRX, Deubiquitinating enzyme FAF-X, EC:3.4.19.12, FAM, Fat facets in mammals, Fat facets protein-related, X-linked, USP9, USP9X, Ubiquitin carboxyl-terminal hydrolase 9X, Ubiquitin thioesterase FAF-X, Ubiquitin-specific protease 9, X chromosome, Ubiquitin-specific-processing protease FAF-X, hFAM
배경

Ubiquitin carboxyl-terminal hydrolase 9X is a ~290 kDa protein. Deubiquitinase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. May therefore play an important regulatory role at the level of protein turnover by preventing degradation of proteins through the removal of conjugated ubiquitin. Specifically hydrolyzes 'Lys-11'-, followed by 'Lys-63'-, 'Lys-48'- and 'Lys-6'-linked polyubiquitins chains. Essential component of TGF-beta/BMP signaling cascade. Specifically deubiquitinates monoubiquitinated SMAD4, opposing the activity of E3 ubiquitin-protein ligase TRIM33.

1. Al-Hakim, AK. et al. (2008) The Biochemical journal 411, 249-60. PMID: 18254724
2. Dupont, S. et al. (2009) Cell 136, 123-35. PMID: 19135894
3. Grou, CP. et al. (2012) The Journal of biological chemistry 287, 12815-27. PMID: 22371489
4. Zhao, Y. et al. (2015) The EMBO journal 34, 1687-703. PMID: 25944111
5. Zhang, Y. et al. (2018) The Biochemical journal 475, 1507-1522. PMID: 29626158
6. Paudel, P. et al. (2019) Proceedings of the National Academy of Sciences of the United States of America 116, 7288-7297. PMID: 30914461
7. Wang, P. et al. (2023) The Journal of biological chemistry 299, 105055. PMID: 37454738
9. Vong, QP. et al. (2005) Science (New York, N.Y.) 310, 1499-504. PMID: 16322459
참고사항 For research use only
이미지
참고 문헌
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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