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| 카탈로그 번호 | HX922012 |
|---|---|
| 설명 |
Recombinant Human VCP Protein, N-His (HX922012) expressed in E. coli, spanning Gly125-Ile371. Purity: >90% by SDS-PAGE.
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | P55072 |
| 단백질 길이 | Gly125-Ile371 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 예측 분자량 | 29.83 kDa |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | Transitional endoplasmic reticulum ATPase, Valosin-containing protein, 15S Mg(2+)-ATPase p97 subunit, TER ATPase, VCP |
| 배경 | Transitional endoplasmic reticulum ATPase (VCP) is a ~89 kDa protein. Necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. Involved in the formation of the transitional endoplasmic reticulum (tER). The transfer of membranes from the endoplasmic reticulum to the Golgi apparatus occurs via 50-70 nm transition vesicles which derive from part-rough, part-smooth transitional elements of the endoplasmic reticulum (tER). Vesicle budding from the tER is an ATP-dependent process. The ternary complex containing UFD1, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. 1. Teng, Y. et al. (2015) Biochemical pharmacology 97, 518-530. PMID: 26265139 2. Kadowaki, H. et al. (2015) Cell reports 13, 944-56. PMID: 26565908 3. Turakhiya, A. et al. (2018) Molecular cell 70, 906-919.e7. PMID: 29804830 4. Gwon, Y. et al. (2021) Science (New York, N.Y.) 372, eabf6548. PMID: 34739333 5. Meerang, M. et al. (2011) Nature cell biology 13, 1376-82. PMID: 22020440 6. Acs, K. et al. (2011) Nature structural & molecular biology 18, 1345-50. PMID: 22120668 7. Mosbech, A. et al. (2012) Nature structural & molecular biology 19, 1084-92. PMID: 23042605 8. Davis, EJ. et al. (2012) Nature structural & molecular biology 19, 1093-100. PMID: 23042607 9. Fielden, J. et al. (2020) Nature communications 11, 1274. PMID: 32152270 10. Krastev, DB. et al. (2022) Nature cell biology 24, 62-73. PMID: 35013556 |
| 참고사항 | For research use only. |
SDS-PAGE for Recombinant Human VCP Protein, N-His
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