中文
English
한국어
日本語
Español
Français
Русский
| 카탈로그 번호 | HY071012 |
|---|---|
| 설명 |
Recombinant Human XRCC5/Ku80 Protein, N-His (HY071012) expressed in E. coli, spanning Leu384-Ile732. Purity: >90% by SDS-PAGE.
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | P13010 |
| 단백질 길이 | Leu384-Ile732 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 예측 분자량 | 41.89 kDa |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | Lupus Ku autoantigen protein p86, XRCC5, Thyroid-lupus autoantigen, Ku86, ATP-dependent DNA helicase 2 subunit 2, CTC85, G22P2, DNA repair protein XRCC5, 86 kDa subunit of Ku antigen, TLAA, Nuclear factor IV, CTC box-binding factor 85 kDa subunit, ATP-dependent DNA helicase II 80 kDa subunit, CTCBF, Ku80, X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining), X-ray repair cross-complementing protein 5 |
| 배경 | DNA repair protein Ku80 (XRCC5) is a ~82 kDa protein. DNA-binding protein critical for the DNA damage response, specifically in repairing double-strand breaks (DSBs) via the classical non-homologous end joining (NHEJ) pathway. It forms a heterodimer with XRCC6 (Ku70), creating the Ku70:Ku80 heterodimer (Ku complex), which serves as a DNA end-binding complex. It primarily binds DSBs and recruits essential repair factors, assembling the core long-range NHEJ complex to facilitate the alignment and ligation of broken DNA ends. This pathway ensures the rapid repair of cytotoxic and mutagenic DSBs and contributes to the generation of diversity in T-cell receptors and antibodies through mechanisms such as V(D)J recombination. Likely acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), catalyzing the beta-elimination of the 5'-deoxyribose-5-phosphate at abasic sites near DSBs. 1. Walker, JR. et al. (2001) Nature 412, 607-14. PMID: 11493912 2. Chen, S. et al. (2021) Nature 593, 294-298. PMID: 33854234 3. Chaplin, AK. et al. (2021) Molecular cell 81, 3400-3409.e3. PMID: 34352203 4. West, RB. et al. (1998) Molecular and cellular biology 18, 5908-20. PMID: 9742108 5. Roberts, SA. et al. (2010) Nature 464, 1214-7. PMID: 20383123 6. Tuteja, N. et al. (1994) The EMBO journal 13, 4991-5001. PMID: 7957065 7. Maekawa, T. et al. (2018) Nucleic acids research 46, 4487-4504. PMID: 29490055 8. Willis, DM. et al. (2002) The Journal of biological chemistry 277, 37280-91. PMID: 12145306 9. Chung, U. et al. (1996) The Journal of biological chemistry 271, 8593-8. PMID: 8621488 10. Morchikh, M. et al. (2017) Molecular cell 67, 387-399.e5. PMID: 28712728 |
| 참고사항 | For research use only. |
SDS-PAGE for Recombinant Human XRCC5/Ku80 Protein, N-His
+86-027-65523339
중국 우한시 심둔사로 666번지 C동, 우한, 430206
