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| 카탈로그 번호 | MW310011 |
|---|---|
| 설명 |
Recombinant Mouse GPC3/Glypican 3 Protein, C-His (MW310011) expressed in Insect Cells, spanning Met1-Ser553.
Highlights
|
| 발현 시스템 | Insect cells |
| 등록번호 | Q8CFZ4 |
| 단백질 길이 | Met1-Ser553 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Mus musculus (Mouse) |
| 성질 | Recombinant |
| 테스트된 응용 분야 | WB |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >95% as determined by SDS-PAGE. |
| 예측 분자량 | 63.44 kDa |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | Glypican-3, Glypican-3 alpha subunit, Glypican-3 beta subunit, Gpc3 |
| 배경 | Glypican-3 (GPC3/Glypican 3) is a ~65 kDa protein. Cell surface proteoglycan. Negatively regulates the hedgehog signaling pathway when attached via the GPI-anchor to the cell surface by competing with the hedgehog receptor PTC1 for binding to hedgehog proteins. Binding to the hedgehog protein SHH triggers internalization of the complex by endocytosis and its subsequent lysosomal degradation. Positively regulates the canonical Wnt signaling pathway by binding to the Wnt receptor Frizzled and stimulating the binding of the Frizzled receptor to Wnt ligands. Positively regulates the non-canonical Wnt signaling pathway. 1. Capurro, MI. et al. (2008) Developmental cell 14, 700-11. PMID: 18477453 2. Bhave, VS. et al. (2013) The American journal of pathology 183, 153-9. PMID: 23665349 3. Song, HH. et al. (2005) The Journal of biological chemistry 280, 2116-25. PMID: 15537637 4. Paine-Saunders, S. et al. (2000) Developmental biology 225, 179-87. PMID: 10964473 5. Grisaru, S. et al. (2001) Developmental biology 231, 31-46. PMID: 11180950 6. Ng, A. et al. (2009) Developmental biology 335, 208-15. PMID: 19733558 |
| 참고사항 | For research use only. |
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