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| 카탈로그 번호 | VK499012 |
|---|---|
| 설명 |
Recombinant Nipah henipavirus M/Matrix Protein, N-His (VK499012) expressed in E. coli, spanning Met1-Gly352. Purity: >90% by SDS-PAGE.
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | Q9IK90 |
| 단백질 길이 | Met1-Gly352 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Nipah virus (NiV) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 형태 | Liquid |
| 저장 완충액 | 0.01M PBS pH 7.4 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | Matrix protein, Protein M, M protein |
| 배경 | Matrix protein is a ~39 kDa protein. Plays a crucial role in virion assembly and budding. Forms a shell at the inner face of the plasma membrane. Transits through the host nucleus before gaining the functional ability to localize and bud from the plasma membrane. Mediates together with fusion protein the incorporation of the glycoprotein to the viral particles. Also participates in the inhibition of the host interferon type I antiviral response by interacting with and thereby inhibiting host TRIM6. _x005F_x005F_x000D_ _x005F_x005F_x000D_1. Ciancanelli, MJ. et al. (2006) Journal of virology 80, 12070-8. PMID: 17005661 _x005F_x005F_x000D_
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2. Patch, JR. et al. (2007) Virology journal 4, 1. PMID: 17204159 _x005F_x005F_x000D_
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3. Patch, JR. et al. (2008) Virology journal 5, 137. PMID: 19000317 _x005F_x005F_x000D_
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4. Wang, YE. et al. (2010) PLoS pathogens 6, e1001186. PMID: 21085610 _x005F_x005F_x000D_
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5. Johnston, GP. et al. (2017) Journal of virology 91. PMID: 28250132 _x005F_x005F_x000D_
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6. Bharaj, P. et al. (2016) PLoS pathogens 12, e1005880. PMID: 27622505 _x005F_x005F_x000D_ |
| 참고사항 | For research use only. |
SDS-PAGE for Recombinant Nipah henipavirus M/Matrix Protein, N-His
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