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| 카탈로그 번호 | JN094012 |
|---|---|
| 설명 |
Recombinant Porphyromonas gingivalis fimA Protein, N-His (JN094012) expressed in E. coli, spanning Asn20-Trp383. Purity: >90% by SDS-PAGE.
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | B2RH54 |
| 단백질 길이 | Asn20-Trp383 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Porphyromonas gingivalis |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 예측 분자량 | 41.62 kDa |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | Major fimbrium subunit FimA type-1, Fimbrillin, Fimbrilin, Major fimbrial subunit protein type I, fimA |
| 배경 | Major fimbrium subunit FimA type-1 is a ~41 kDa protein. Structural subunit of the major fimbriae. These long, filamentous pili are attached to the cell surface; they mediate biofilm formation, adhesion onto host cells and onto other bacteria that are part of the oral microbiome. They play an important role in the invasion of periodontal tissues. Fimbriae and their constituents are major virulence factors. FimA proteins from different strains have highly divergent sequences, and this has been used for classification. 1. Umemoto, T. et al. (2003) Journal of periodontology 74, 119-22. PMID: 12593606 2. Shoji, M. et al. (2004) Molecular microbiology 52, 1513-25. PMID: 15165251 3. Nishiyama, SI. et al. (2007) Microbiology (Reading, England) 153, 1916-1925. PMID: 17526848 4. Wang, M. et al. (2007) Journal of immunology (Baltimore, Md. : 1950) 179, 2349-58. PMID: 17675496 5. Nagano, K. et al. (2010) Journal of dental research 89, 903-8. PMID: 20530728 6. Xu, Q. et al. (2016) Cell 165, 690-703. PMID: 27062925 7. Kadowaki, T. et al. (1998) The Journal of biological chemistry 273, 29072-6. PMID: 9786913 8. Nagano, K. et al. (2013) Molecular oral microbiology 28, 392-403. PMID: 23809984 |
| 참고사항 | For research use only. |
Detected by Anti-P.gingivalis fimA/Fimbrilin Antibody (123-123-10) (Cat No.: JN847013) in indirect ELISA.
SDS-PAGE for Porphyromonas gingivalis fimA Recombinant Protein (N-His)
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