Anti-Human 5'-Nucleotidase/CD73 Reference Antibody (Ansipastobart, RUO) (HB209156)

Name: Anti-Human 5'-Nucleotidase/CD73 Reference Antibody (Ansipastobart, RUO)
Brand: abinScience
SKU: HB209156
Price: 176 USD
Availability: InStock

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100ug
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개요
이미지
참고 문헌
데이터시트
SDS

개요

카탈로그 번호

HB209156

설명

Ansipastobart (HB209156) is a research-grade recombinant antibody targeting CD73. Produced in mammalian cells with native-like glycosylation.

Highlights

●Research Grade — For PK/PD studies, assay development, and ADA research.
●Native Glycosylation — Mammalian expression ensures native-like patterns.

종 반응성

Human

응용 분야

ELISA, Bioactivity: FCM, Functional assay, Research in vivo, WB

숙주 종

Human

클론성

Monoclonal

이소타입

IgG1-lambda2

테스트된 응용 분야

타겟

5'-NT, 5'-deoxynucleotidase, 5'-nucleotidase, CD73, EC:3.1.3.35, EC:3.1.3.5, EC:3.1.3.89, EC:3.1.3.91, EC:3.1.3.99, Ecto-5'-nucleotidase, IMP-specific 5'-nucleotidase, NT5, NT5E, NTE, Thymidylate 5'-phosphatase

내독소 수준

< 10 EU/mg

순도

>95% as determined by SDS-PAGE.

정제

Protein A/G purified from cell culture supernatant.

등록번호

P21589

형태

Liquid

저장 완충액

0.01M PBS pH 7.4.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

대체 이름

2829250-32-8

배경

5'-nucleotidase (CD73/NT5E) is a ~63 kDa protein. Catalyzes the hydrolysis of nucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside, with AMP being the preferred substrate. Shows a preference for ribonucleotide monophosphates over their equivalent deoxyribose forms. Other substrates include IMP, UMP, GMP, CMP, dAMP, dCMP, dTMP, NAD and NMN.

1. Garavaglia, S. et al. (2012) The Biochemical journal 441, 131-41. PMID: 21933152

2. Heuts, DP. et al. (2012) Chembiochem : a European journal of chemical biology 13, 2384-91. PMID: 22997138

3. Knapp, K. et al. (2012) Structure (London, England : 1993) 20, 2161-73. PMID: 23142347

4. Fausther, M. et al. (2014) PloS one 9, e98568. PMID: 24887587

5. Scaletti, E. et al. (2021) Purinergic signalling 17, 693-704. PMID: 34403084

참고사항

For research use only. Not suitable for clinical or therapeutic use.

Isotype Control

Human IgG1, lambda Isotype Control antibody (HyHEL-10) (HP248107)

이미지

SDS-PAGE

SDS-PAGE for Research Grade Ansipastobart
Bioactivity

Detects recombinant CD73 (Catalog No: HB209011) in indirect ELISA.
Western blot

Western blot analysis was performed using anti-CD73 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human CD73 (Catalog No: HB209011)
Lane 2: Negative control