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| 카탈로그 번호 | HB016046 |
|---|---|
| 종 반응성 | Human |
| 응용 분야 | ELISA, Bioactivity: FACS, Functional assay, Research in vivo |
| 숙주 종 | Human |
| 클론성 | Monoclonal |
| 이소타입 | IgG1-kappa |
| 클론 ID | GSK2618960 |
| 생물학적 활성 | GSK2618960 (10-100 ug/mL; 10 h) potently induces dose-dependent CD4 + T cell proliferation in PBMCs [2]. GSK2618960 binds specifically to monocyte-derived DCs, but not to uncultured primary DCs, NK cells, or B cells from fresh PBMCs [2]. GSK2618960 (50 ug/mL; 7 days) enhances the differentiation of DCs and upregulates their activation marker (CD83, CD86 and CD209) expression in PBMCs [2]. |
| 발현 시스템 | Mammalian cells |
| 테스트된 응용 분야 | FCM |
| 타겟 | CD127, CDw127, IL-7 receptor subunit alpha, IL-7R subunit alpha, IL-7R-alpha, IL-7RA, IL7R, Interleukin-7 receptor subunit alpha |
| 내독소 수준 | < 10 EU/mg |
| 순도 | >95% purity as determined by SDS-PAGE. |
| 정제 | Protein A/G purified from cell culture supernatant. |
| 등록번호 | P16871 |
| 형태 | Liquid |
| 저장 완충액 | 0.01M PBS pH 7.4 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | GSK2618960 |
| 배경 | Interleukin-7 receptor subunit alpha (CD127/IL7R) is a ~51 kDa protein. Receptor for interleukin-7. Also acts as a receptor for thymic stromal lymphopoietin (TSLP). |
| 참고 문헌 | 1. Ellis J, et al. Anti-IL-7 receptor α monoclonal antibody (GSK2618960) in healthy subjects - a randomized, double-blind, placebo-controlled study. Br J Clin Pharmacol. 2019;85(2):304-315. [HB016046] 2. Liao K, et al. Characterization of the robust humoral immune response to GSK2618960, a humanized anti-IL-7 receptor monoclonal antibody, observed in healthy subjects in a Phase 1 study. PLoS One. 2021;16(3):e0249049. Published 2021 Mar 23. [HB016046] |
| 참고사항 | For research use only. Not suitable for clinical or therapeutic use. |
| Isotype Control | Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507) Human IgG1 (E356D/M358L), kappa Recombinant Isotype Control Antibody (HyHEL-10) (HV080013) Anti-LASV GPC Recombinant Antibody (Fab 37.7) (VK453013) |
Detects Human CD127/IL7R in indirect ELISAs.
SDS-PAGE for Research Grade Anti-Human CD127/IL7R (GSK2618960)
Flow-cytometry using APC anti-human CD127 antibody. 293T transfected cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD127 monoclonal antibody (Catalog HB016046, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry using PE anti-human CD127 antibody. 293T transfected cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD127 monoclonal antibody (Catalog HB016046, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
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