Anti-Human MCAM/CD146 Reference Antibody (Abx-Ma1, RUO) (HW484026)

Name: Anti-Human MCAM/CD146 Reference Antibody (Abx-Ma1, RUO)
Brand: abinScience
SKU: HW484026
Price: 176 USD
Availability: InStock

데이터시트 제품 공유 정보 복사

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100ug
1mg
5mg
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25mg
50mg
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개요
이미지
참고 문헌
데이터시트
SDS

개요

카탈로그 번호	HW484026
종 반응성	Human
응용 분야	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
숙주 종	Human
클론성	Monoclonal
이소타입	IgG1
클론 ID	Abx-Ma1
발현 시스템	Mammalian cells
테스트된 응용 분야	FCM
타겟	CD146, Melanoma cell adhesion molecule, S-endo 1 endothelial-associated antigen, Melanoma-associated antigen A32, MUC18, MCAM, Melanoma-associated antigen MUC18, Cell surface glycoprotein MUC18, Cell surface glycoprotein P1H12
내독소 수준	< 10 EU/mg
순도	>95% purity as determined by SDS-PAGE.
정제	Protein A/G purified from cell culture supernatant.
등록번호	P43121
형태	Liquid
저장 완충액	0.01M PBS pH 7.4 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.
안정성 및 저장	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
대체 이름	C3.19.1, Abx-Ma1
배경	Cell surface glycoprotein MUC18 (CD146/MCAM) is a ~71 kDa protein. Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as a surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.
참고 문헌	1. Braeuer RR, et al. Transcriptional control of melanoma metastasis: the importance of the tumor microenvironment. Semin Cancer Biol. 2011 Apr;21(2):83-8. [HW484026]
참고사항	For research use only. Not suitable for clinical or therapeutic use.
Isotype Control	Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)

이미지

SEC-HPLC

SEC-HPLC detection for Research Grade Anti-Human CD146/MUC18/MCAM Antibody (Abx-Ma1).
Bioactivity

Detects CD146/MUC18/MCAM in indirect ELISAs.
SDS-PAGE

SDS-PAGE for Research Grade Anti-Human CD146/MUC18/MCAM Antibody (Abx-Ma1).
Flow CytoMetry

Flow-cytometry using APC anti-human CD146 antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD146 monoclonal antibody (Catalog: HW484026, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using FITC anti-human CD146 antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD146 monoclonal antibody (Catalog: HW484026, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using PE anti-human CD146 antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD146 monoclonal antibody (Catalog: HW484026, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.