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| 카탈로그 번호 | HV612076 |
|---|---|
| 설명 |
Anti-Human CD276/B7-H3 Reference Antibody (DS-5573a, RUO) (HV612076) is a human monoclonal antibody detecting CD276/B7-H3.
|
| 종 반응성 | Human |
| 응용 분야 | ELISA, Bioactivity: FCM, Functional assay, Research in vivo |
| 숙주 종 | Human |
| 클론성 | Monoclonal |
| 이소타입 | IgG |
| 클론 ID | DS-5573a |
| 생물학적 활성 | DS-5573a (1 ug/mL) binds to the IgC1 and IgC2 domains of human B7-H3 expressed in transfected CHO-K1 cells [1]. The K d value of DS-5573a binding to recombinant human 4IgB7-H3 is 1.8 nM, and the K d value of its binding to recombinant human 2IgB7-H3 is 11 nM [1]. DS-5573a (0.01-1000 ng/mL; 4 h) exerts potent, B7-H3-dependent antibody-dependent cell-mediated cytotoxicity against NCI-H322, MDA-MB-231 and COLO205 cells via human peripheral blood mononuclear cells (PBMC), while exhibits only minimal activity against B7-H3-negative CCRF-CEM cells [1]. DS-5573a (1-10000 ng/mL; 3 h) exerts B7-H3-dependent antibody-dependent cellular phagocytosis (ADCP) activity against NCI-H322, MDA-MB-231 and COLO205 cells via human monocyte-derived macrophages, but shows no activity against B7-H3-negative CCRF-CEM cells [1]. Cell Cytotoxicity Assay [1] Cell Line: NCI-H322, MDA-MB-231, and COLO205 cells Concentration: 0.01, 0.1, 1, 10, 100, 1000 ng/mL Incubation Time: 4 h Result: Exerted potent, B7-H3-dependent ADCC activity against NCI-H322, MDA-MB-231, and COLO205 cells via human PBMCs. DS-5573a (0.003-3 mg/kg; i.p.; once a week; 5 weeks) exhibits dose-dependent, statistically significant antitumor activity against MDA-MB-231 breast adenocarcinoma in female SCID mice, with significant efficacy observed at doses as low as 0.03 mg/kg and maximal activity at 0.3 mg/kg and 3 mg/kg [1]. |
| 발현 시스템 | Mammalian cells |
| 성질 | Recombinant |
| 타겟 | 4Ig-B7-H3, B7 homolog 3, B7-H3, B7H3, CD276, CD276 antigen, Costimulatory molecule |
| 내독소 수준 | < 10 EU/mg as determined by LAL method. |
| 순도 | >95% as determined by SDS-PAGE. |
| 정제 | Protein A/G purified from cell culture supernatant. |
| 등록번호 | Q5ZPR3 |
| 형태 | Liquid |
| 저장 완충액 | 0.01M PBS pH 7.4. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | DS-5573a, DS5573a, DS5573a |
| 배경 | CD276 antigen (B7-H3) is a ~57 kDa protein. May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. CD276 is the therapeutic target of omburtamab (clinical). |
| 참고 문헌 | 1. Nagase-Zembutsu A, et al. Development of DS-5573a: A novel afucosylated mAb directed at B7-H3 with potent antitumor activity. Cancer Sci. 2016;107(5):674-681. [HV612076] |
| 참고사항 | For research use only. Not suitable for clinical or therapeutic use. |
| Isotype Control | Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507) |
SDS-PAGE for Research Grade Anti-Human CD276/B7-H3 (DS-5573a).
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