Anti-Human CD3 epsilon Reference Antibody (IOR-T3, RUO) (HY057096)

Name: Anti-Human CD3 epsilon Reference Antibody (IOR-T3, RUO)
Brand: abinScience
SKU: HY057096
Price: 370 USD
Availability: InStock

데이터시트 제품 공유 정보 복사

가격(USD):

사양:

100ug
1mg
5mg
10mg
25mg
50mg
100mg

수량:

문의하기

개요
이미지
참고 문헌
데이터시트
SDS

개요

카탈로그 번호	HY057096
종 반응성	Human
응용 분야	ELISA, Bioactivity: FCM, Functional assay, Research in vivo
숙주 종	Human
클론성	Monoclonal
이소타입	IgG
클론 ID	IOR-T3
생물학적 활성	IOR-T3 (1 h) potently binds to CD3 on human PBMCs, with equivalent antigen-binding capacity to its chimeric variants T3q and T3q(Ala=Ala) [1]. IOR-T3 (70-100 ug/mL; 7 days) does not inhibit the growth of human non-small-cell lung carcinoma H125 cells when used at 100 ug/mL for 7 days [2]. Cell Proliferation Assay [2] Cell Line: human non-small-cell lung carcinoma H125 cells Concentration: 70, 90, 100 ug/mL Incubation Time: 7 days Result: Showed no inhibitory effect on H125 cell growth, with cell protein content remaining at 100% of control levels.
타겟	CD3E, CD3e, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, T3E
내독소 수준	< 10 EU/mg
순도	>95% as determined by SDS-PAGE.
정제	Protein A/G purified from cell culture supernatant.
등록번호	P07766
형태	Liquid
저장 완충액	0.01M PBS pH 7.4. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.
안정성 및 저장	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
대체 이름	IOR-T3, IORT3, IORT3
배경	T-cell surface glycoprotein CD3 epsilon chain (CD3E) is a ~23 kDa protein. Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD247/CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. CD3E ITAM phosphorylation creates docking sites for the protein kinase ZAP70 leading to ZAP70 phosphorylation and its conversion into a catalytically active enzyme. CD3E is the therapeutic target of blinatumomab (Blincyto). 1. Gimferrer, I. et al. (2004) Journal of immunology (Baltimore, Md. : 1950) 173, 2262-70. PMID: 15294938 2. de Saint Basile, G. et al. (2004) The Journal of clinical investigation 114, 1512-7. PMID: 15546002 3. Barber, EK. et al. (1989) Proceedings of the National Academy of Sciences of the United States of America 86, 3277-81. PMID: 2470098 4. Du, J. et al. (2025) Cell 188, 4025-4042.e20. PMID: 40592325 5. Soudais, C. et al. (1993) Nature genetics 3, 77-81. PMID: 8490660
참고 문헌	1. Hinojosa LE, et al. Construction of a recombinant non-mitogenic anti-human CD3 antibody. Hybridoma (Larchmt). 2010;29(2):115-124. [HY057096] 2. Suarez Pestana E, et al. Growth inhibition of human lung adenocarcinoma cells by antibodies against epidermal growth factor receptor and by ganglioside GM3: involvement of receptor-directed protein tyrosine phosphatase(s). Br J Cancer. 1997;75(2):213-220. [HY057096]
참고사항	For research use only. Not suitable for clinical or therapeutic use.