Anti-Human CD47 Reference Antibody (CC-90002, RUO) (HC359076)

Name: Anti-Human CD47 Reference Antibody (CC-90002, RUO)
Brand: abinScience
SKU: HC359076
Price: 176 USD
Availability: InStock

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100ug
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개요
이미지
참고 문헌
데이터시트
SDS

개요

카탈로그 번호	HC359076
종 반응성	Human
응용 분야	ELISA, Bioactivity: FCM, Functional assay, Research in vivo
숙주 종	Human
클론성	Monoclonal
이소타입	IgG4
클론 ID	CC-90002
생물학적 활성	CC-90002 has a high affinity for binding to CD47 with a subnanomolar dissociation constant (K d) value [1]. CC-90002 can block the CD47-SIRPa interaction with IC 50 value in the subnanomolar range [1]. CC-90002 has inhibitory effect in concentration-dependent in the ALL and AML cell lines [1]. CC-90002 shows significant dose-dependent antitumor activity and significant tumor regression in tumor xenografts models [1].
테스트된 응용 분야	FCM
타겟	Antigenic surface determinant protein OA3, CD47, IAP, Integrin-associated protein, Leukocyte surface antigen CD47, MER6, Protein MER6
내독소 수준	< 10 EU/mg
순도	>95% as determined by SDS-PAGE.
정제	Protein A/G purified from cell culture supernatant.
등록번호	Q08722
형태	Liquid
저장 완충액	0.01M PBS pH 7.4. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.
안정성 및 저장	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
대체 이름	CC-90002, INBRX-103, CC90002, DHG17620
배경	Leukocyte surface antigen CD47 (MER6) is a ~35 kDa protein. Adhesive protein that mediates cell-to-cell interactions. Acts as a receptor for thrombospondin THBS1 and as modulator of integrin signaling through the activation of heterotrimeric G proteins. Involved in signal transduction, cardiovascular homeostasis, inflammation, apoptosis, angiogenesis, cellular self-renewal, and immunoregulation. Plays a role in modulating pulmonary endothelin EDN1 signaling. Modulates nitrous oxide (NO) signaling, in response to THBS1, hence playing a role as a pressor agent, supporting blood pressure. CD47 is the therapeutic target of magrolimab (clinical). 1. Latour, S. et al. (2001) Journal of immunology (Baltimore, Md. : 1950) 167, 2547-54. PMID: 11509594 2. Piccio, L. et al. (2005) Blood 105, 2421-7. PMID: 15383453 3. Isenberg, JS. et al. (2009) The Journal of biological chemistry 284, 1116-25. PMID: 19004835 4. Lindberg, FP. et al. (1993) The Journal of cell biology 123, 485-96. PMID: 7691831 5. Gao, AG. et al. (1996) The Journal of biological chemistry 271, 21-4. PMID: 8550562 6. Rogers, NM. et al. (2017) Cardiovascular research 113, 15-29. PMID: 27742621 7. Ghimire, K. et al. (2020) Cells 9. PMID: 32679764
참고 문헌	1. Rama Krishna Narla, et al. The humanized anti-CD47 monclonal antibody, CC-90002, has antitumor activity in vitro and in vivo. [HC359076] 2. Zhongxing Jiang, et al. Targeting CD47 for cancer immunotherapy. J Hematol Oncol. 2021 Oct 30;14(1):180. [HC359076]
참고사항	For research use only. Not suitable for clinical or therapeutic use.
Isotype Control	Human IgG4 (S228P) Recombinant Isotype Control Antibody (13R4) (HP298207) Human IgG4 (S228P & L235E) Recombinant Isotype Control Antibody (HyHEL-10) (HP614107) Human IgG4 (S228P) Recombinant Isotype Control Antibody (SAA0184) (HP298107)

이미지

Bioactivity

Detects Human CD47/MER6 in indirect ELISAs.
SDS-PAGE

SDS-PAGE for Research Grade Anti-Human CD47/MER6 Antibody (CC-90002).
Flow CytoMetry

Flow-cytometry using APC anti-human CD47 antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD47 monoclonal antibody (Catalog: HC359076, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using PE anti-human CD47 antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD47 monoclonal antibody (Catalog: HC359076, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using FITC anti-human CD47 antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD47 monoclonal antibody (Catalog: HC359076, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.