Anti-Human CD9 Reference Antibody (AT14-012, RUO) (HB984016)

Name: Anti-Human CD9 Reference Antibody (AT14-012, RUO)
Brand: abinScience
SKU: HB984016
Price: 176 USD
Availability: InStock

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개요
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참고 문헌
데이터시트
SDS

개요

카탈로그 번호	HB984016
설명	Anti-Human CD9 (AT14-012) (HB984016) is a research-grade recombinant antibody targeting CD9. Produced in mammalian cells with native-like glycosylation. Highlights ●Research Grade — For PK/PD studies, assay development, and ADA research. ●Native Glycosylation — Mammalian expression ensures native-like patterns.
종 반응성	Human
응용 분야	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
숙주 종	Human
클론성	Monoclonal
이소타입	IgG1-kappa
클론 ID	AT14-012
발현 시스템	Mammalian cells
테스트된 응용 분야	FCM
타겟	Motility-related protein, Leukocyte antigen MIC3, TSPAN29, Cell growth-inhibiting gene 2 protein, CD9, MIC3, p24, Tspan-29, MRP-1, 5H9 antigen, Tetraspanin-29, CD9 antigen
내독소 수준	< 10 EU/mg
순도	>95% purity as determined by SDS-PAGE.
정제	Protein A/G purified from cell culture supernatant.
등록번호	P21926
형태	Liquid
저장 완충액	0.01M PBS pH 7.4 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.
안정성 및 저장	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
대체 이름	AT14-012, AT14012, AT14012
배경	CD9 antigen is a ~25 kDa protein. Integral membrane protein associated with integrins, which regulates different processes, such as sperm-egg fusion, platelet activation and aggregation, and cell adhesion. Present at the cell surface of oocytes and plays a key role in sperm-egg fusion, possibly by organizing multiprotein complexes and the morphology of the membrane required for the fusion. In myoblasts, associates with CD81 and PTGFRN and inhibits myotube fusion during muscle regeneration. In macrophages, associates with CD81 and beta-1 and beta-2 integrins, and prevents macrophage fusion into multinucleated giant cells specialized in ingesting complement-opsonized large particles. Also prevents the fusion between mononuclear cell progenitors into osteoclasts in charge of bone resorption. 1. Higginbottom, A. et al. (2003) Biochemical and biophysical research communications 311, 208-14. PMID: 14575715 2. Nakazawa, Y. et al. (2008) Blood 112, 1730-9. PMID: 18541721 3. Ikeyama, S. et al. (1993) The Journal of experimental medicine 177, 1231-7. PMID: 8478605 4. Takeda, Y. et al. (2003) The Journal of cell biology 161, 945-56. PMID: 12796480 5. Masellis-Smith, A. et al. (1994) Journal of immunology (Baltimore, Md. : 1950) 152, 2768-77. PMID: 7511626
참고사항	For research use only. Not suitable for clinical or therapeutic use.
Isotype Control	Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)

이미지

Bioactivity

Detects Recombinant Human CD9 Protein, C-His (Cat No.: HB984021) in indirect ELISA.
SDS-PAGE

SDS-PAGE for Research Grade Anti-Human CD9 (AT14-012).
Flow cytometry

Flow-cytometry using APC anti-human CD9 antibody. HCT-116 cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD9 monoclonal antibody (Catalog HB984016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow cytometry

Flow-cytometry using PE anti-human CD9 antibody. HCT-116 cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD9 monoclonal antibody (Catalog HB984016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow cytometry

Flow-cytometry using FITC anti-human CD9 antibody. HCT-116 cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD9 monoclonal antibody (Catalog HB984016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.