Anti-Human VEGFA Reference Antibody (Bevacizumab, RUO) (HB941026)

Name: Anti-Human VEGFA Reference Antibody (Bevacizumab, RUO)
Brand: abinScience
SKU: HB941026
Price: 176 USD
Availability: InStock

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100ug
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개요
이미지
참고 문헌
데이터시트
SDS

개요

카탈로그 번호	HB941026
설명	Bevacizumab (HB941026) is a research-grade recombinant antibody targeting Vascular endothelial growth factor A. Produced in mammalian cells with native-like glycosylation. Highlights ●Research Grade — For PK/PD studies, assay development, and ADA research. ●Native Glycosylation — Mammalian expression ensures native-like patterns.
종 반응성	Human
응용 분야	ELISA, Bioactivity: FCM, Functional assay, Research in vivo
숙주 종	Human
클론성	Monoclonal
이소타입	IgG1-kappa
테스트된 응용 분야	FCM
타겟	L-VEGF, N-VEGF, VEGF, VEGFA, VPF, Vascular endothelial growth factor A, long form, Vascular permeability factor
내독소 수준	< 10 EU/mg
순도	>95% as determined by SDS-PAGE.
정제	Protein A/G purified from cell culture supernatant.
등록번호	P15692
형태	Liquid
저장 완충액	0.01M PBS pH 7.4. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.
안정성 및 저장	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
대체 이름	12-IgG1, F(ab)-12IgG1, Fab-12IgG1, rhuMAb-VEGF, ABP215, 216974-75-3
배경	Vascular endothelial growth factor A, long form (VEGFA) is a ~43 kDa protein. Participates in the induction of key genes involved in the response to hypoxia and in the induction of angiogenesis such as HIF1A. Involved in protecting cells from hypoxia-mediated cell death. VEGFA is the therapeutic target of bevacizumab (Avastin). 1. Katsman, M. et al. (2022) Cells 11. PMID: 35455969
참고사항	For research use only. Not suitable for clinical or therapeutic use.
Isotype Control	Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)

이미지

SEC-HPLC

SEC-HPLC detection for Research Grade Bevacizumab.
Bioactivity

Detects Human VEGF165 in indirect ELISAs.
Flow Cytometry

Flow-cytometry using anti-human VEGFA antibody. U937 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an anti-human VEGFA monoclonal antibody (Catalog HB941026, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE

SDS-PAGE for Research Grade Bevacizumab
Flow CytoMetry

Flow-cytometry using FITC anti-human VEGFA antibody. U87 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human VEGFA monoclonal antibody (Catalog: HB941026, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.