Anti-Human IGF-I R/IGF1R Reference Antibody (Ganitumab, RUO) (HY353026)

Name: Anti-Human IGF-I R/IGF1R Reference Antibody (Ganitumab, RUO)
Brand: abinScience
SKU: HY353026
Price: 176 USD
Availability: InStock

데이터시트 제품 공유 정보 복사

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100ug
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개요
이미지
참고 문헌
데이터시트
SDS

개요

카탈로그 번호	HY353026
설명	Ganitumab (HY353026) is a research-grade recombinant antibody targeting CD221. Produced in mammalian cells with native-like glycosylation. Highlights ●Research Grade — For PK/PD studies, assay development, and ADA research. ●Native Glycosylation — Mammalian expression ensures native-like patterns.
종 반응성	Human
응용 분야	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
숙주 종	Human
클론성	Monoclonal
이소타입	IgG1-kappa
발현 시스템	Mammalian cells
테스트된 응용 분야	FCM
타겟	CD221, Insulin-like growth factor 1 receptor, IGF1R, Insulin-like growth factor I receptor, IGF-I receptor
내독소 수준	< 10 EU/mg
순도	>95% purity as determined by SDS-PAGE.
정제	Protein A/G purified from cell culture supernatant.
등록번호	P08069
형태	Liquid
저장 완충액	0.01M PBS pH 7.4 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.
안정성 및 저장	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
대체 이름	AMG479, 905703-97-1
배경	Insulin-like growth factor 1 receptor (CD221/IGF1R) is a ~154 kDa protein. Receptor tyrosine kinase which mediates actions of insulin-like growth factor 1 (IGF1). Binds IGF1 with high affinity and IGF2 and insulin (INS) with a lower affinity. The activated IGF1R is involved in cell growth and survival control. IGF1R is crucial for tumor transformation and survival of malignant cell. Ligand binding activates the receptor kinase, leading to receptor autophosphorylation, and tyrosines phosphorylation of multiple substrates, that function as signaling adapter proteins including, the insulin-receptor substrates (IRS1/2), Shc and 14-3-3 proteins.
참고사항	For research use only. Not suitable for clinical or therapeutic use.

이미지

Bioactivity

SEC-HPLC detection for Research Grade Ganitumab.
Flow Cytometry

Flow-cytometry using anti-human IGFIR antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IGFIR monoclonal antibody (Catalog HY353026, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human IGFIR antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human IGFIR monoclonal antibody (Catalog HY353026) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE

SDS-PAGE for Anti-Human IGF-I R/IGF1R Reference Antibody (Ganitumab, RUO)
Flow CytoMetry

Flow-cytometry using APC anti-human CD221 antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD221 monoclonal antibody (Catalog: HY353026, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using PE anti-human CD221 antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD221 monoclonal antibody (Catalog: HY353026, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.