Anti-Human MICA/B Reference Antibody (IPH4301, RUO) (HS894016)

Name: Anti-Human MICA/B Reference Antibody (IPH4301, RUO)
Brand: abinScience
SKU: HS894016
Price: 176 USD
Availability: InStock

데이터시트 제품 공유 정보 복사

가격(USD):

사양:

100ug
1mg
5mg
10mg
25mg
50mg
100mg

수량:

문의하기

개요
이미지
참고 문헌
데이터시트
SDS

개요

카탈로그 번호

HS894016

종 반응성

Human

응용 분야

ELISA, Bioactivity: FACS, Functional assay, Research in vivo

숙주 종

Human

클론성

Monoclonal

이소타입

IgG1-kappa

클론 ID

IPH4301

생물학적 활성

IPH4301 induces death of tumor cells expressing MICA/B in vitro via antibody-dependent cellular cytotoxicity and antibody-dependent cellular phagocytosis [1]. IPH4301 blocks the binding of MICA/B to NKG2D, and restores the expression and function of NKG2D on primary human NK cells and T cells [1]. IPH4301 reverses the immunosuppressive effect induced by M2 macrophages and restores the ADCC activity of autologous NK cells against MICA-expressing tumor cell lines [1]. IPH4301-derived PEG-G5.NH2-FITC-DOTA(Gd)-Monalizumab/IPH4301 nanoprobe can efficiently bind to NK-92MI cells. Its binding efficiency and in vitro MRI signal increase with elevated concentrations; besides, it upregulates IFN-γ secretion of NK-92MI cells, enhances the cytotoxicity against MDA-MB-231 triple-negative breast cancer cells and induces tumor cell apoptosis [2].

발현 시스템

Mammalian cells

타겟

MICA, MIC-A, MHC class I polypeptide-related sequence A, PERB11.1, MHC class I polypeptide-related sequence B, MIC-B, MICB, PERB11.2

내독소 수준

< 10 EU/mg

순도

>95% purity as determined by SDS-PAGE.

정제

Protein A/G purified from cell culture supernatant.

등록번호

Q29983, Q29980

형태

Liquid

저장 완충액

0.01M PBS pH 7.4

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:2000-1:20000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

대체 이름

IPH4301

배경

MHC class I polypeptide-related sequence A (MICA/MICB) is a ~42 kDa protein. Widely expressed membrane-bound protein which acts as a ligand to stimulate an activating receptor KLRK1/NKG2D, expressed on the surface of essentially all human natural killer (NK), gammadelta T and CD8 alphabeta T-cells. Up-regulated in stressed conditions, such as viral and bacterial infections or DNA damage response, serves as signal of cellular stress, and engagement of KLRK1/NKG2D by MICA triggers NK-cells resulting in a range of immune effector functions, such as cytotoxicity and cytokine production.

1. Steinle, A. et al. (2001) Immunogenetics 53, 279-87. PMID: 11491531

2. Sutherland, CL. et al. (2002) Journal of immunology (Baltimore, Md. : 1950) 168, 671-9. PMID: 11777960

3. Bauer, S. et al. (1999) Science (New York, N.Y.) 285, 727-9. PMID: 10426993

참고 문헌

1. Morel A, et al. Abstract 1491: IPH4301, an antibody targeting MICA and MICB exhibits potent cytotoxic activity and immunomodulatory properties for the treatment of cancer. Cancer Res. 2016 Jul;76(14 Suppl):1491. [HS894016]
2. Wang Z, et al. Preliminary study on PEG-G5.NH2-FITC-DOTA(Gd)-Monalizumab/IPH4301 probe. Biotechnol Bull. 2023;10. [HS894016]

참고사항

For research use only. Not suitable for clinical or therapeutic use.

이미지