Anti-Human CD28 Reference Antibody (Pegrizeprument, RUO) (HY422056)

Name: Anti-Human CD28 Reference Antibody (Pegrizeprument, RUO)
Brand: abinScience
SKU: HY422056
Price: 370 USD
Availability: InStock

데이터시트 제품 공유 정보 복사

가격(USD):

사양:

100ug
1mg
5mg
10mg
25mg
50mg
100mg

수량:

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개요
이미지
참고 문헌
데이터시트
SDS

개요

카탈로그 번호

HY422056

설명

Pegrizeprument (HY422056) is a research-grade recombinant antibody targeting CD28. Produced in mammalian cells with native-like glycosylation.

Highlights

●Research Grade — For PK/PD studies, assay development, and ADA research.
●Native Glycosylation — Mammalian expression ensures native-like patterns.

종 반응성

Human

응용 분야

ELISA, Bioactivity: FACS, Functional assay, Research in vivo, WB

숙주 종

Chimeric

클론성

Monoclonal

이소타입

Immunoglobulin Fab VH-G1CH1h_L-kappa

발현 시스템

Mammalian cells

테스트된 응용 분야

타겟

CD28, TP44, T-cell-specific surface glycoprotein CD28

내독소 수준

< 10 EU/mg

순도

>95% purity as determined by SDS-PAGE.

정제

Protein A/G purified from cell culture supernatant.

등록번호

P10747

형태

Liquid

저장 완충액

0.01M PBS pH 7.4

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

대체 이름

2829316-24-5

배경

T-cell-specific surface glycoprotein CD28 is a ~25 kDa protein. Receptor that plays a role in T-cell activation, proliferation, survival and the maintenance of immune homeostasis. Functions not only as an amplifier of TCR signals but delivers unique signals that control intracellular biochemical events that alter the gene expression program of T-cells. Stimulation upon engagement of its cognate ligands CD80 or CD86 increases proliferation and expression of various cytokines in particular IL2 production in both CD4(+) and CD8(+) T-cell subsets. Mechanistically, ligation induces recruitment of protein kinase C-theta/PRKCQ and GRB2 leading to NF-kappa-B activation via both PI3K/Akt-dependent and -independent pathways. In conjunction with TCR/CD3 ligation and CD40L costimulation, enhances the production of IL4 and IL10 in T-cells.

1. Gimmi, CD. et al. (1991) Proceedings of the National Academy of Sciences of the United States of America 88, 6575-9. PMID: 1650475

2. Raab, M. et al. (1995) Proceedings of the National Academy of Sciences of the United States of America 92, 8891-5. PMID: 7568038

3. Skånland, SS. et al. (2014) The Biochemical journal 460, 399-410. PMID: 24665965

4. Collins, AV. et al. (2002) Immunity 17, 201-10. PMID: 12196291

5. Wu, H. et al. (2022) Structure (London, England : 1993) 30, 803-812.e5. PMID: 35397202

6. Kong, KF. et al. (2011) Nature immunology 12, 1105-12. PMID: 21964608

7. Blotta, MH. et al. (1996) Journal of immunology (Baltimore, Md. : 1950) 156, 3133-40. PMID: 8617933

참고사항

For research use only. Not suitable for clinical or therapeutic use.

이미지

SDS-PAGE

SDS-PAGE for Research Grade Pegrizeprument.
Western blot

Western blot analysis was performed using anti-CD28 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human CD28 (Catalog No: HY422011)
Lane 2: Negative control